环状 RNA-ABCB10 通过上调 ROCK1 促进鼻咽癌的生长和转移。
Circ-ABCB10 promotes growth and metastasis of nasopharyngeal carcinoma by upregulating ROCK1.
机构信息
Department of Otolaryngology, Lianyungang First People's Hospital, Lianyungang, China.
出版信息
Eur Rev Med Pharmacol Sci. 2020 Dec;24(23):12208-12215. doi: 10.26355/eurrev_202012_24011.
OBJECTIVE
Nasopharyngeal carcinoma (NPC) is one of the most ordinary head and neck tumors. This study aims to uncover the role of circ-ABCB10 functions in the progression of NPC.
PATIENTS AND METHODS
The circ-ABCB10 expression was detected in paired NPC patients' tissue samples and cell lines. The role of circ-ABCB10 in NPC proliferation was identified through proliferation assay, Ethynyl deoxyuridine (EdU) assay and colony formation assay. Next, the role of circ-ABCB10 in NPC metastasis was measured through wound healing assay and transwell assay. Finally, the underlying mechanism was further uncovered through Western blot assay and real-time quantitative polymerase chain reaction (RT-qPCR).
RESULTS
It was found that circ-ABCB10 expression was significantly higher in NPC tissues than that in adjacent samples. In addition, cell proliferation, migration and invasion of NPC were promoted through overexpression of circ-ABCB10 in vitro. Results of further experiments revealed that ROCK1 was upregulated via overexpression of circ-ABCB10 in NPC.
CONCLUSIONS
This study indicated that circ-ABCB10 promoted NPC cell proliferation and metastasis via upregulating ROCK1 in vitro.
目的
鼻咽癌（NPC）是最常见的头颈部肿瘤之一。本研究旨在揭示 circ-ABCB10 在 NPC 进展中的作用。
患者和方法
检测了配对的 NPC 患者组织样本和细胞系中的 circ-ABCB10 表达。通过增殖试验、Ethynyl deoxyuridine（EdU）试验和集落形成试验鉴定 circ-ABCB10 在 NPC 增殖中的作用。接下来，通过划痕愈合试验和 Transwell 试验测量 circ-ABCB10 在 NPC 转移中的作用。最后，通过 Western blot 试验和实时定量聚合酶链反应（RT-qPCR）进一步揭示潜在机制。
结果
发现 circ-ABCB10 在 NPC 组织中的表达明显高于相邻样本。此外，体外过表达 circ-ABCB10 可促进 NPC 细胞的增殖、迁移和侵袭。进一步实验结果表明，ROCK1 通过 circ-ABCB10 的过表达在上调 NPC 中上调。
结论
本研究表明，circ-ABCB10 通过体外上调 ROCK1 促进 NPC 细胞的增殖和转移。
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