使用基于 TRIzol 的方法从动物组织中提取高纯度 DNA。

High-purity DNA extraction from animal tissue using picking in the TRIzol-based method.

机构信息

Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang City, Liaoning Province 110866, China.

Beijing Animal Disease Prevention & Control Center, Beijing 102609, China.

出版信息

Biotechniques. 2021 Mar;70(3):186-190. doi: 10.2144/btn-2020-0142. Epub 2020 Dec 18.

DOI:10.2144/btn-2020-0142

PMID:33337248

Abstract

TRIzol is used for the extraction of RNA, DNA and proteins from tissues or cells. Here, we present a simple picking method to extract DNA from tissues using TRIzol. Spectrophotometric analysis showed that the 260/280 and 260/230 nm optical density ratio of the picking method's DNA is ideal and better than that obtained by the classic TRIzol method. Gel electrophoresis showed that there was no RNA contamination, and the DNA had not degraded. DNA extracted by the picking method had the same performance in restriction enzyme digestion and quantitative PCR as that obtained by the traditional method. Viral DNA in the infected tissue was also obtained. This modified method facilitates various molecular biology assays.

摘要

TRIzol 用于从组织或细胞中提取 RNA、DNA 和蛋白质。在这里，我们提出了一种使用 TRIzol 从组织中提取 DNA 的简单挑取方法。分光光度分析表明，该挑取方法的 DNA 的 260/280 和 260/230nm 光密度比值理想，优于经典 TRIzol 方法获得的值。凝胶电泳表明没有 RNA 污染，并且 DNA 没有降解。通过挑取方法提取的 DNA 在限制酶消化和定量 PCR 中的性能与传统方法获得的 DNA 相同。还获得了感染组织中的病毒 DNA。该改进方法方便了各种分子生物学检测。