（4,7）-吉马二烯醇合酶来自和的分子机制差异。

Mechanistic divergence between (4,7)-germacra-(1(10),5)-dien-11-ol synthases from and .

机构信息

Kekulé-Institute of Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagk-Strasse 1, 53121 Bonn, Germany.

Department of Plant Sciences, University of Tennessee, 2431 Joe Johnson Drive, Knoxville, TN 37996-4561, USA.

出版信息

Org Biomol Chem. 2021 Jan 21;19(2):370-374. doi: 10.1039/d0ob02361b.

DOI:10.1039/d0ob02361b

PMID:33337456

Abstract

The main product of DpTPS9 from the social amoeba Dictyostelium purpureum was identified as (4S,7R)-germacra-(1(10)E,5E)-dien-11-ol that is also known as an intermediate of bacterial geosmin synthase, but the experimentally verified cyclisation mechanisms differ. Together with the low sequence identity this points to convergent evolution. The functionality of selected residues in DpTPS9 was investigated via site-directed mutagenesis experiments.

摘要

从粘菌紫色喇叭手 Dictyostelium purpureum 中分离到的 DpTPS9 的主要产物被鉴定为(4S,7R)-germacra-(1(10)E,5E)-dien-11-ol，它也是细菌土臭素合酶的中间产物，但实验验证的环化机制不同。再加上低的序列同一性，这表明了趋同进化。通过定点突变实验研究了 DpTPS9 中选定残基的功能。