循环肿瘤细胞的血小板伪装：转移性前列腺癌患者的 ExPeCT 随机对照试验结果。
Platelet cloaking of circulating tumour cells in patients with metastatic prostate cancer: Results from ExPeCT, a randomised controlled trial.
机构信息
Department of Histopathology and Morbid Anatomy, Trinity Translational Medicine Institute, Trinity College Dublin, Dublin, Ireland.
Department of Histopathology, Cork University Hospital, Cork, Ireland.
出版信息
PLoS One. 2020 Dec 18;15(12):e0243928. doi: 10.1371/journal.pone.0243928. eCollection 2020.
BACKGROUND
Circulating tumour cells (CTCs) represent a morphologically distinct subset of cancer cells, which aid the metastatic spread. The ExPeCT trial aimed to examine the effectiveness of a structured exercise programme in modulating levels of CTCs and platelet cloaking in patients with metastatic prostate cancer.
METHODS
Participants (n = 61) were randomised into either standard care (control) or exercise arms. Whole blood was collected for all participants at baseline (T0), three months (T3) and six months (T6), and analysed for the presence of CTCs, CTC clusters and platelet cloaking. CTC data was correlated with clinico-pathological information.
RESULTS
Changes in CTC number were observed within group over time, however no significant difference in CTC number was observed between groups over time. Platelet cloaking was identified in 29.5% of participants. A positive correlation between CTC number and white cell count (WCC) was observed (p = 0.0001), in addition to a positive relationship between CTC clusters and PSA levels (p = 0.0393).
CONCLUSION
The presence of platelet cloaking has been observed in this patient population for the first time, in addition to a significant correlation between CTC number and WCC.
TRIAL REGISTRATION
ClincalTrials.gov identifier NCT02453139.
背景
循环肿瘤细胞 (CTC) 是形态上不同于癌细胞的一个亚群，它们有助于肿瘤的转移扩散。ExPeCT 试验旨在研究结构性运动方案对转移性前列腺癌患者 CTC 水平和血小板包裹的调节作用。
方法
将 61 名参与者随机分配到标准护理（对照组）或运动组。所有参与者在基线（T0）、三个月（T3）和六个月（T6）时采集全血，以检测 CTC、CTC 簇和血小板包裹的存在。对 CTC 数据与临床病理信息进行相关性分析。
结果
观察到组内 CTC 数量随时间的变化，但组间随时间的 CTC 数量无显著差异。在 29.5%的参与者中发现了血小板包裹。观察到 CTC 数量与白细胞计数（WCC）之间存在正相关（p = 0.0001），此外，CTC 簇与 PSA 水平之间也存在正相关关系（p = 0.0393）。
结论
首次在该患者人群中观察到血小板包裹的存在，同时 CTC 数量与 WCC 之间存在显著相关性。
试验注册
ClinicalTrials.gov 标识符 NCT02453139。
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