Institute of Laboratory Medicine, Sichuan Provincial Key Laboratory for Human, Disease Gene Study, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610054, China.
Anesthesia Operation Center, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, China.
Exp Eye Res. 2021 Feb;203:108392. doi: 10.1016/j.exer.2020.108392. Epub 2020 Dec 15.
The laser-induced choroidal neovascularization (CNV) mouse model, as the most classic animal model of age-related macular degeneration (AMD), has been widely used. We designed a hand-held mouse holder to optimize mouse fixation in the laser-induced CNV modelling process, which was inconvenient until now. This study aimed to evaluate the effectiveness of our in-house hand-held mouse holder design in the laser-induced CNV mouse modelling process.
Six ophthalmic residents were invited to perform laser-induced CNV mouse modelling by hand or using the holder. We compared the learning time of residents and their physical and mental fatigue with the two methods. In addition, we compared the parameters of CNV modelling with two methods by a skilled operator, including the time of photocoagulation, induction rate and uniformity of CNV lesions.
In the learning phase, the average learning time to master the modelling method was significantly shortened by utilizing the holder. The fatigue in the operation process was quantified to a level from 0 to 4, and the physical fatigue by using holder (0.8 ± 0.3) was lower than by hand (2.6 ± 0.4), and the mental fatigue was relieved from 2.3 ± 0.5 to 0.4 ± 0.3. On the other hand, the skilled operator can significantly shorten the time of laser photocoagulation from 146.7 ± 36.0 s to 63.6 + 5.7 s and improve the success rate of modelling from 50.0% ± 8.3%-87.5% ± 6.7% by using a holder compared to hand. In addition, the standard error of the mean (SEM) of the distance between the CNV lesion and the optic nerve (ON) and the distance between each lesion was reduced.
This hand-held mouse holder could optimize the setting and conditions of laser-induced CNV mouse modelling by improving the learning curve, reducing fatigue, shortening the time for photocoagulation, improving the success rate and consistency of laser-induced lesions.
激光诱导脉络膜新生血管（CNV）小鼠模型是年龄相关性黄斑变性（AMD）最经典的动物模型，已被广泛应用。我们设计了一种手持式小鼠固定器，以优化激光诱导 CNV 建模过程中的小鼠固定，这在以前是不方便的。本研究旨在评估我们自制的手持式小鼠固定器在激光诱导 CNV 小鼠建模过程中的有效性。
邀请六名眼科住院医师通过手动或使用固定器进行激光诱导 CNV 小鼠建模。我们比较了住院医师使用两种方法的学习时间以及他们的身心疲劳程度。此外，由一名熟练的操作员比较了两种方法的 CNV 建模参数，包括光凝时间、诱导率和 CNV 病变的均匀性。
在学习阶段，使用固定器显著缩短了掌握建模方法的平均学习时间。操作过程中的疲劳程度量化为 0 到 4 级，使用固定器的身体疲劳（0.8 ± 0.3）低于手动（2.6 ± 0.4），精神疲劳从 2.3 ± 0.5 减轻到 0.4 ± 0.3。另一方面，熟练操作员使用固定器可以将激光光凝时间从 146.7 ± 36.0 秒显著缩短至 63.6 ± 5.7 秒，并将建模成功率从 50.0% ± 8.3%提高至 87.5% ± 6.7%。此外，还降低了 CNV 病变与视神经（ON）之间的距离和每个病变之间的距离的平均值标准误差（SEM）。
这种手持式小鼠固定器可以通过改善学习曲线、减少疲劳、缩短光凝时间、提高激光诱导病变的成功率和一致性，来优化激光诱导 CNV 小鼠建模的设置和条件。
