香鱼（Plecoglossus altivelis）尿皮质素的特性：一种内分泌因子在单核细胞/巨噬细胞调节中的作用。
Characterization of ayu (Plecoglossus altivelis) urocortin: The function of an endocrine factor in monocyte/macrophage regulation.
作者信息
Jiang Rui, Lu Xin-Jiang, Lu Jian-Fei, Chen Jiong
机构信息
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, 315211, China; Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo, 315211, China; Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo, 315211, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, 315211, China; Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo, 315211, China; Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo University, Ningbo, 315211, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), China.
出版信息
Dev Comp Immunol. 2021 Apr;117:103978. doi: 10.1016/j.dci.2020.103978. Epub 2020 Dec 16.
Urocortin (UCN) is a hormone in the hypothalamic-pituitary-adrenal axis that is expressed in various immune cells. However, the function of teleost UCN in the immune system remains unclear. In this study, we cloned the cDNA sequence of UCN from ayu Plecoglossus altivelis (PaUCN). Sequence and phylogenetic tree analyses showed that PaUCN clustered within the fish UCN 1 group and was most related to the rainbow trout (Oncorhynchus mykiss) UCN. PaUCN was expressed in all tested tissues and its expression increased in the liver, spleen, head kidney, and gill upon Vibrio anguillarum infection. Mature PaUCN protein (mPaUCN) treatment affected the phagocytosis and bacterial killing of monocytes/macrophages (MO/MФ). mPaUCN reduced pro-inflammatory cytokine expression in MO/MФ, which was partially mediated via interaction with ayu interleukin-6. mPaUCN reduced bacterial load and increased the survival of V. anguillarum-infected ayu. Overall, UCN as an endocrine factor regulates the immune response of ayu after infection by activating MO/MФ, thus contributing to enhance fish survival.
尿皮质素（UCN）是下丘脑 - 垂体 - 肾上腺轴中的一种激素，在多种免疫细胞中表达。然而，硬骨鱼UCN在免疫系统中的功能仍不清楚。在本研究中，我们从香鱼（Plecoglossus altivelis）中克隆了UCN的cDNA序列（PaUCN）。序列和系统发育树分析表明，PaUCN聚集在鱼类UCN 1组内，与虹鳟（Oncorhynchus mykiss）UCN关系最为密切。PaUCN在所有测试组织中均有表达，鳗弧菌感染后，其在肝脏、脾脏、头肾和鳃中的表达增加。成熟的PaUCN蛋白（mPaUCN）处理影响单核细胞/巨噬细胞（MO/MФ）的吞噬作用和细菌杀伤能力。mPaUCN降低了MO/MФ中促炎细胞因子的表达，这部分是通过与香鱼白细胞介素-6相互作用介导的。mPaUCN降低了细菌载量，并提高了鳗弧菌感染的香鱼的存活率。总体而言，UCN作为一种内分泌因子，通过激活MO/MФ来调节香鱼感染后的免疫反应，从而有助于提高鱼类的存活率。
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