Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China.
Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China; Chengdu University of Traditional Chinese Medicine, Chengdu 611137, Sichuan, China.
Genomics. 2021 Jan;113(1 Pt 1):193-204. doi: 10.1016/j.ygeno.2020.12.025. Epub 2020 Dec 15.
Non-coding RNAs appear to be involved in the regulation of the nervous system. However, no competing endogenous RNA (ceRNA) network related to PM damage in the hippocampal function has yet been constructed. Herein, we used whole-transcriptome sequencing technology to systematically study the ceRNA network in rat hippocampi after PM exposure. We identified 100 circRNAs, 67 lncRNAs, 28 miRNAs, and 539 mRNAs and constructed the most comprehensive ceRNA network to date, to our knowledge. Gene Ontology and KEGG analyses showed that the network molecules are involved in synapses, neural projections, and neural development and involve signal pathways such as the synaptic vesicle cycle. Finally, the expression of the differentially expressed RNAs confirmed by quantitative real-time PCR was consistent with the sequencing data. This study systematically dissected the ceRNA atlas related to cognitive memory function in the hippocampal tissue of PM-exposed rats for the first time, to our knowledge, and promotes the development of potential new treatments for cognitive impairment.
非编码 RNA 似乎参与了神经系统的调节。然而，目前尚未构建与 PM 损伤相关的海马功能的竞争性内源性 RNA (ceRNA) 网络。在此，我们使用全转录组测序技术系统研究了 PM 暴露后大鼠海马中的 ceRNA 网络。我们鉴定了 100 个 circRNAs、67 个 lncRNAs、28 个 miRNAs 和 539 个 mRNAs，并构建了迄今为止最全面的 ceRNA 网络，据我们所知。GO 和 KEGG 分析表明，网络分子参与突触、神经投射和神经发育，并涉及突触囊泡循环等信号通路。最后，通过定量实时 PCR 验证的差异表达 RNA 的表达与测序数据一致。这项研究首次系统地剖析了 PM 暴露大鼠海马组织中与认知记忆功能相关的 ceRNA 图谱，据我们所知，这促进了认知障碍潜在新治疗方法的发展。
