Cooper Medical School, Camden, NJ, United States.
Cancer Treat Res Commun. 2021;26:100274. doi: 10.1016/j.ctarc.2020.100274. Epub 2020 Dec 10.
Immune infiltration in neuroblastoma (NBL) has been associated with clinical outcome. However, the diversity of distinct immune subpopulations that comprise immune infiltrates in NBL has not been examined. To this end, the present study investigated the immunological landscape of NBL tumors and its clinical significance. CIBERSORTx, an established RNA deconvolution algorithm, was used to impute immune cell proportions from 153 primary NBL tumors. Associations between immune proportions and overall/event-free survival were analyzed by Kaplan-Meier curves and evaluated using log-rank test. Of the 22 subpopulations imputed, M2 macrophages were the most abundant subtype in NBL tumors. Furthermore, monocytes, CD4+ naïve T cells, and CD4+ activated memory T cells were significantly associated with survival. Altogether, the findings suggest differences amongst certain immune cell subsets comprising NBL tumor infiltration and these differences may be important determinants of prognosis.
神经母细胞瘤（NBL）中的免疫浸润与临床结局相关。然而，构成 NBL 免疫浸润的不同免疫亚群的多样性尚未被研究。为此，本研究调查了 NBL 肿瘤的免疫学特征及其临床意义。CIBERSORTx 是一种已建立的 RNA 去卷积算法，用于从 153 个原发性 NBL 肿瘤中推断免疫细胞比例。通过 Kaplan-Meier 曲线和对数秩检验分析免疫比例与总生存/无事件生存的关系。在推断的 22 个亚群中，M2 巨噬细胞是 NBL 肿瘤中最丰富的亚型。此外，单核细胞、CD4+幼稚 T 细胞和 CD4+激活记忆 T 细胞与生存显著相关。总之，这些发现表明构成 NBL 肿瘤浸润的某些免疫细胞亚群之间存在差异，这些差异可能是预后的重要决定因素。
