Departament de Ciències Mèdiques Bàsiques, IRBLleida, Universitat de Lleida, 25198 Lleida, Spain.
Int J Mol Sci. 2020 Dec 16;21(24):9574. doi: 10.3390/ijms21249574.
Hcm1 is a member of the forkhead transcription factor family involved in segregation, spindle pole dynamics, and budding in . Our group described the role of Hcm1 in mitochondrial biogenesis and stress resistance, and in the cellular adaptation to mitochondrial respiratory metabolism when nutrients decrease. Regulation of Hcm1 activity occurs at the protein level, subcellular localization, and transcriptional activity. Here we report that the amount of protein increased in the G1/S transition phase when the factor accumulated in the nucleus. In the G2/M phases, the Hcm1 amount decreased, and it was translocated outside the nucleus with a network-like localization. Preparation of highly purified mitochondria by a sucrose gradient density demonstrated that Hcm1 colocalized with mitochondrial markers, inducing expression of , a mitochondrial encoded subunit of cytochrome oxidase, in the G2/M phases. Taken together, these results show a new localization of Hcm1 and suggest that it acts as a mitochondrial transcription factor regulating the metabolism of this organelle.
Hcm1 是叉头框转录因子家族的成员，参与分离、纺锤极动力学和出芽作用。我们的小组描述了 Hcm1 在线粒体生物发生和应激抗性中的作用，以及在营养物质减少时细胞适应线粒体呼吸代谢的作用。Hcm1 活性的调节发生在蛋白质水平、亚细胞定位和转录活性上。在这里，我们报告了当因子积累在核内时，蛋白质的量在 G1/S 转换期增加。在 G2/M 期，Hcm1 的量减少，并与核外的网络状定位一起被转运。通过蔗糖梯度密度制备高度纯化的线粒体表明，Hcm1 与线粒体标记物共定位，在 G2/M 期诱导细胞色素氧化酶的线粒体编码亚基 的表达。总之，这些结果显示了 Hcm1 的新定位，并表明它作为一种线粒体转录因子调节该细胞器的代谢。
