Future Industries Institute, University of South Australia, Adelaide 5000, Australia.
Centre for Aquatic Animal Health & Vaccines. Department of Primary Industries Parks Water & Environment Tasmania, 165 Westbury Road, Prospect, Tasmania 7250, Australia.
Biointerphases. 2020 Dec 18;15(6):061012. doi: 10.1116/6.0000511.
A drug-eluting coating applied onto biomedical devices and implants is an appropriate way to ensure that an inhibitory concentration of antimicrobial drugs is present at the device surface, thus preventing surface colonization and subsequent biofilm formation. In this study, a thin polymer coating was applied to materials, and it acted as a drug-delivery reservoir capable of surface delivery of the antifungal drug fluconazole to amounts up to 21 μg/cm. The release kinetics into aqueous solution were quantified by UV spectroscopy and conformed to the Ritger-Peppas and Korsmeyer-Peppas model. Complementary microbiological assays were used to determine effectiveness against Candida albicans attachment and biofilm formation, and against the control heptylamine plasma polymer coating without drug loading, on which substantial fungal growth occurred. Fluconazole release led to marked antifungal activity in all assays, with log 1.6 reduction in CFUs/cm. Cell viability assays and microscopy revealed that fungal cells attached to the fluconazole-loaded coating remained rounded and did not form hyphae and biofilm. Thus, in vitro screening results for fluconazole-releasing surface coatings showed efficacy in the prevention of the formation of Candida albicans biofilm.
将药物洗脱涂层应用于生物医学设备和植入物是一种确保在设备表面存在抑制浓度的抗菌药物的有效方法，从而防止表面定植和随后的生物膜形成。在这项研究中，将薄聚合物涂层施加到材料上，其充当药物输送储库，能够将抗真菌药物氟康唑表面输送到高达 21μg/cm 的量。通过紫外光谱法对水相中的释放动力学进行定量，并符合 Ritger-Peppas 和 Korsmeyer-Peppas 模型。互补的微生物学测定用于确定对白色念珠菌附着和生物膜形成的有效性，以及对没有药物负载的对照庚基胺等离子体聚合物涂层的有效性，在该涂层上发生了大量真菌生长。氟康唑的释放导致所有测定中均表现出明显的抗真菌活性，CFU/cm 的对数减少了 1.6。细胞活力测定和显微镜检查显示，附着在氟康唑负载涂层上的真菌细胞保持圆形，并且不形成菌丝和生物膜。因此，氟康唑释放表面涂层的体外筛选结果显示出预防白色念珠菌生物膜形成的功效。
