类器官移植和植入后血管化整体可视化的方法。

A Method for Organoid Transplantation and Whole-Mount Visualization of Post-Engraftment Vascularization.

机构信息

School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ, USA.

出版信息

Methods Mol Biol. 2021;2258:259-272. doi: 10.1007/978-1-0716-1174-6_17.

Abstract

As the field of organoid development matures, the need to transplant organoids to evaluate and characterize their functionality grows. Decades of research developing islet organoid transplantation for the treatment of type 1 diabetes can contribute substantially to accelerating diverse tissue organoid transplantation. Biomaterials-based organoid delivery methods offer the potential to maximize organoid survival and engraftment. In this protocol, we describe a vasculogenic degradable hydrogel vehicle and a method to deliver organoids to intraperitoneal tissue. Further, we describe a method to fluorescently label and image functional vasculature within the graft as a tool to investigate organoid engraftment.

摘要

随着类器官发育领域的成熟，需要将类器官移植到评估和描述其功能的需求也在增长。数十年的胰岛类器官移植研究为加速多种组织类器官移植提供了重要支持。基于生物材料的类器官输送方法具有最大化类器官存活和植入的潜力。在本方案中，我们描述了一种血管生成可降解水凝胶载体和一种将类器官递送到腹腔组织的方法。此外，我们还描述了一种荧光标记和成像移植物内功能性血管的方法，作为研究类器官植入的工具。