Innovation Team of Small Animal Infectious Disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, 200241, China.
Innovation Team of Small Animal Infectious Disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Shanghai, 200241, China.
Vet Microbiol. 2021 Feb;253:108950. doi: 10.1016/j.vetmic.2020.108950. Epub 2020 Dec 8.
A novel duck picornavirus, designated as duck/AH15/CHN/2015, was isolated and identified from Cherry Valley ducks with short beak and dwarfism syndrome in 2015 in Anhui province of China. Duck/AH15/CHN/2015 has the highest degree of amino acid sequence identity (approximately 43 %) with duck hepatitis A viruses (DHAV) Complete genome analysis revealed that duck/AH15/CHN/2015 possesses a typical picornavirus-like genomic organization, 5' UTR-L-P1 (VP0-VP3-VP1)-P2 (2A1-2A2- 2B-2C)-P3 (3A-3B-3C-3D)-3'UTR-poly (A). The 5'UTR contains a potential type IV internal ribosome entry site, while a conserved "barbell"-like structure is found at the 3'UTR, which is similar to DHAV. Compared to the closest related DHAVs, two unrelated 2A proteins were predicted in duck/AH15/CHN/2015, while three unrelated 2A proteins were presented in DHAVs. Based on the amino acid identity comparison and phylogenetic analysis of P1, 2C, and 3CD (3C and 3D), duck/AH15/CHN/2015 was closely related to but distinct from DHAVs, and it was proposed to be a member of a novel species in the genus Avihepatovirus of the family Picornaviridae.
一种新型鸭源小 RNA 病毒，被命名为鸭/AH15/CHN/2015，于 2015 年在中国安徽省从樱桃谷鸭中分离鉴定，该病毒可引起短喙矮小型综合征。鸭/AH15/CHN/2015 与鸭甲型肝炎病毒（DHAV）的氨基酸序列同源性最高（约为 43%）。全基因组分析显示，鸭/AH15/CHN/2015 具有典型的小 RNA 病毒基因组结构，5'UTR-L-P1（VP0-VP3-VP1）-P2（2A1-2A2-2B-2C）-P3（3A-3B-3C-3D）-3'UTR-多聚（A）。5'UTR 含有一个潜在的 IV 型内部核糖体进入位点，而 3'UTR 存在保守的“哑铃”样结构，类似于 DHAV。与最接近的相关 DHAV 相比，鸭/AH15/CHN/2015 中预测存在两种不相关的 2A 蛋白，而 DHAV 中存在三种不相关的 2A 蛋白。基于 P1、2C 和 3CD（3C 和 3D）的氨基酸同一性比较和系统进化分析，鸭/AH15/CHN/2015 与 DHAV 密切相关，但又有区别，建议将其归为小 RNA 病毒科禽肝病毒属的一个新型种。
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