Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Szigeti út 12., 7624, Pecs, Hungary.
Hungarian Nature Research Society, Ajka, Hungary.
Arch Virol. 2021 Sep;166(9):2627-2632. doi: 10.1007/s00705-021-05167-y. Epub 2021 Jul 13.
In this study, a novel picornavirus (perchPV/M9/2015/HUN, GenBank accession no. MW590713) was detected in eight (12.9%) out of 62 faecal samples collected from three (Perca fluviatilis, Sander lucioperca, and Ameiurus melas) out of 13 freshwater fish species tested and genetically characterized using viral metagenomics and RT-PCR methods. The complete genome of perchPV/M9/2015/HUN is 7,741 nt long, excluding the poly(A) tail, and has the genome organization 5'UTRP1(VP0-VP3-VP1)/P2(2A-2A-2B-2C)/P3(3A-3B-3C-3D)/3'UTR-poly(A). The P1, 2C, and 3CD proteins had 41.4%, 38.1%, and 47.3% amino acid sequence identity to the corresponding proteins of Wenling lepidotrigla picornavirus (MG600079), eel picornavirus (NC_022332), and Wenling pleuronectiformes picornavirus (MG600098), respectively, as the closest relatives in the genus Potamipivirus. PerchPV/M9/2015/HUN represents a potential novel fish-origin species in an unassigned genus in the family Picornaviridae.
在这项研究中，使用病毒宏基因组学和 RT-PCR 方法，从 13 种淡水鱼类中检测到了 3 种（欧洲河鲈、梭鲈和斑点叉尾鮰）的 62 份粪便样本中，有 8 份（12.9%）存在新型微小核糖核酸病毒（perchPV/M9/2015/HUN，GenBank 登录号 MW590713）。perchPV/M9/2015/HUN 的完整基因组长 7741 个核苷酸，不包括 poly(A)尾巴，基因组组织为 5'UTR-P1(VP0-VP3-VP1)/P2(2A-2A-2B-2C)/P3(3A-3B-3C-3D)/3'UTR-poly(A)。P1、2C 和 3CD 蛋白与 Wenling lepidotrigla 微小核糖核酸病毒（MG600079）、鳗微小核糖核酸病毒（NC_022332）和 Wenling 鲽形目微小核糖核酸病毒（MG600098）的相应蛋白的氨基酸序列同源性分别为 41.4%、38.1%和 47.3%，是 Potamipivirus 属中最接近的亲缘病毒。perchPV/M9/2015/HUN 是 Picornaviridae 科未分类属中的一种潜在新型鱼类起源微小核糖核酸病毒。
