来自匈牙利淡水鱼类（梭鲈、欧鳇和红点鲑）的新型微小核糖核酸病毒（微小核糖核酸病毒科）。
Novel picornavirus (family Picornaviridae) from freshwater fishes (Perca fluviatilis, Sander lucioperca, and Ameiurus melas) in Hungary.
机构信息
Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Szigeti út 12., 7624, Pecs, Hungary.
Hungarian Nature Research Society, Ajka, Hungary.
出版信息
Arch Virol. 2021 Sep;166(9):2627-2632. doi: 10.1007/s00705-021-05167-y. Epub 2021 Jul 13.
In this study, a novel picornavirus (perchPV/M9/2015/HUN, GenBank accession no. MW590713) was detected in eight (12.9%) out of 62 faecal samples collected from three (Perca fluviatilis, Sander lucioperca, and Ameiurus melas) out of 13 freshwater fish species tested and genetically characterized using viral metagenomics and RT-PCR methods. The complete genome of perchPV/M9/2015/HUN is 7,741 nt long, excluding the poly(A) tail, and has the genome organization 5'UTRP1(VP0-VP3-VP1)/P2(2A-2A-2B-2C)/P3(3A-3B-3C-3D)/3'UTR-poly(A). The P1, 2C, and 3CD proteins had 41.4%, 38.1%, and 47.3% amino acid sequence identity to the corresponding proteins of Wenling lepidotrigla picornavirus (MG600079), eel picornavirus (NC_022332), and Wenling pleuronectiformes picornavirus (MG600098), respectively, as the closest relatives in the genus Potamipivirus. PerchPV/M9/2015/HUN represents a potential novel fish-origin species in an unassigned genus in the family Picornaviridae.
在这项研究中，使用病毒宏基因组学和 RT-PCR 方法，从 13 种淡水鱼类中检测到了 3 种（欧洲河鲈、梭鲈和斑点叉尾鮰）的 62 份粪便样本中，有 8 份（12.9%）存在新型微小核糖核酸病毒（perchPV/M9/2015/HUN，GenBank 登录号 MW590713）。perchPV/M9/2015/HUN 的完整基因组长 7741 个核苷酸，不包括 poly(A)尾巴，基因组组织为 5'UTR-P1(VP0-VP3-VP1)/P2(2A-2A-2B-2C)/P3(3A-3B-3C-3D)/3'UTR-poly(A)。P1、2C 和 3CD 蛋白与 Wenling lepidotrigla 微小核糖核酸病毒（MG600079）、鳗微小核糖核酸病毒（NC_022332）和 Wenling 鲽形目微小核糖核酸病毒（MG600098）的相应蛋白的氨基酸序列同源性分别为 41.4%、38.1%和 47.3%，是 Potamipivirus 属中最接近的亲缘病毒。perchPV/M9/2015/HUN 是 Picornaviridae 科未分类属中的一种潜在新型鱼类起源微小核糖核酸病毒。
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