Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, UCSI University, No. 1, Jalan Menara Gading, Taman Connaught, Cheras 56000 Kuala Lumpur, Malaysia.
Faculty of Medicine & Health Sciences, UCSI University, No. 1, Jalan Menara Gading, Taman Connaught, Cheras 56000 Kuala Lumpur, Malaysia.
Curr Pharm Biotechnol. 2021;22(7):969-982. doi: 10.2174/1389201021666201218124450.
Recombinant Keratinocyte Growth Factor (rHuKGF) is a therapeutic protein used widely in oral mucositis after chemotherapy in various cancers, stimulating lung morphogenesis and gastrointestinal tract cell proliferation. In this research study, chitosan-rHuKGF polymeric complex was implemented to improve the stability of rHuKGF and used as rejuvenation therapy for the treatment of oral mucositis in cancer patients.
Complexation of rHuKGF with mucoadhesive low molecular weight chitosan to protect rHuKGF from proteolysis and investigate the effect of chitosan-rHuKGF complex on the proliferation rate of FHs 74 Int cells.
The interaction between chitosan and rHuKGF was studied by molecular docking. Malvern ZetaSizer Nano Zs and Fourier-Transform Infrared spectroscopy (FTIR) tests were carried out to characterize the chitosan-rHuKGF complex. In addition, SDS-PAGE was performed to investigate the interaction between chitosan-rHuKGF complex and pepsin. The effect of chitosan-rHuKGF complex on the proliferation rate of FHs 74 Int cells was studied by MTT assay.
Chitosan-rHuKGF complex was formed through the hydrogen bonding proven by the docking studies. A stable chitosan-rHuKGF complex was formed at pH 4.5 and was protected from proteolysis and assessed by SDS PAGE. According to the MTT assay results, chitosan-rHuKGF complex increased the cell proliferation rate of FHs 74 Int cells.
The developed complex improved the stability and the biological function of rHuKGF.
重组角质细胞生长因子（rHuKGF）是一种治疗蛋白，广泛用于各种癌症的化疗后口腔黏膜炎，刺激肺形态发生和胃肠道细胞增殖。在这项研究中，壳聚糖-rHuKGF 聚合物复合物被用于提高 rHuKGF 的稳定性，并用作癌症患者口腔黏膜炎的复兴治疗。
将 rHuKGF 与具有黏膜黏附性的低分子量壳聚糖复合，以保护 rHuKGF 免受蛋白水解，并研究壳聚糖-rHuKGF 复合物对 FHs 74 Int 细胞增殖率的影响。
通过分子对接研究壳聚糖与 rHuKGF 之间的相互作用。使用 Malvern ZetaSizer Nano Zs 和傅里叶变换红外光谱（FTIR）测试对壳聚糖-rHuKGF 复合物进行表征。此外，通过 SDS-PAGE 研究壳聚糖-rHuKGF 复合物与胃蛋白酶的相互作用。通过 MTT 测定法研究壳聚糖-rHuKGF 复合物对 FHs 74 Int 细胞增殖率的影响。
壳聚糖-rHuKGF 复合物是通过对接研究证明的氢键形成的。在 pH 4.5 下形成稳定的壳聚糖-rHuKGF 复合物，并通过 SDS PAGE 评估其免受蛋白水解。根据 MTT 测定结果，壳聚糖-rHuKGF 复合物增加了 FHs 74 Int 细胞的增殖率。
所开发的复合物提高了 rHuKGF 的稳定性和生物学功能。
