建立鉴别寄生家禽的异形唇乳突线虫和鸭冠状唇乳突线虫的常规多重 PCR 方法。
Development of conventional multiplex PCR method for discrimination between Dispharynx nasuta and Cheilospirura hamulosa (Nematoda: Acuariidae) parasitizing poultry.
机构信息
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan.
Department of Pathogenetic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
出版信息
J Vet Med Sci. 2021 Feb 25;83(2):226-229. doi: 10.1292/jvms.20-0664. Epub 2020 Dec 18.
The poultry infections caused by Dispharynx nasuta and Cheilospirura hamulosa nematodes are difficult to be diagnosed by fecal examination because of their egg similarity. In this study, we analyzed DNA sequences of nuclear ribosomal 18S-ITS1-5.8S-ITS2-28S region of D. nasuta and C. hamulosa and developed conventional multiplex PCR method using species-specific primers for discriminating between the two species. The method amplified 455-bp and 319-bp fragments specific to D. nasuta and C. hamulosa, respectively, and did not produce them against the other chicken nematode species, Ascaridia galli, Oxyspirura mansoni, Heterakis gallinarum, Heterakis beramporia, and Heterakis indica, suggesting that the multiplex PCR is sensitive and available for species diagnosis.
由于鼻吻线虫和狭咽唇乳突线虫的卵相似，粪便检查难以诊断它们引起的禽类感染。在本研究中，我们分析了鼻吻线虫和狭咽唇乳突线虫核核糖体 18S-ITS1-5.8S-ITS2-28S 区的 DNA 序列，并使用种特异性引物开发了常规多重 PCR 方法，用于区分这两种线虫。该方法分别扩增了特异性为 455-bp 和 319-bp 的片段，而针对其他鸡线虫种，如鸡蛔虫、曼氏迭宫绦虫、鸡异刺线虫、比翼线虫和鸡异刺线虫，均未产生这些片段，表明该多重 PCR 方法具有较高的灵敏度和种属诊断能力。
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