Brorson I S, Eriksson A M, Leikfoss I S, Vitelli V, Celius E G, Lüders T, Berge T, Harbo H F, Nilsen H, Bos S D
Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
Department of Neurology, Oslo University Hospital Ullevål, Oslo, Norway.
Mult Scler J Exp Transl Clin. 2020 Dec 9;6(4):2055217320978511. doi: 10.1177/2055217320978511. eCollection 2020 Oct-Dec.
Genetic and clinical observations have indicated T cells are involved in MS pathology. There is little insight in how T cells are involved and whether or not these can be used as markers for MS.
Analysis of the gene expression profiles of circulating CD8 T cells of MS patients compared to healthy controls.
RNA from purified CD8 T cells was sequenced and analyzed for differential gene expression. Pathway analyses of genes at several p-value cutoffs were performed to identify putative pathways involved.
We identified 36 genes with significant differential gene expression in MS patients. Four genes reached at least 2-fold differences in expression. The majority of differentially expressed genes was higher expressed in MS patients. Genes associated to MS in GWAS showed enrichment amongst the differentially expressed genes. We did not identify enrichment of specific pathways amongst the differentially expressed genes in MS patients.
CD8 T cells of MS patients show differential gene expression, with predominantly higher activity of genes in MS patients. We do not identify specific biological pathways in our study. More detailed analysis of CD8 T cells and subtypes of these may increase understanding of how T cells are involved in MS.
遗传学和临床观察表明T细胞参与了多发性硬化症（MS）的病理过程。对于T细胞如何参与其中以及它们是否可作为MS的标志物，目前了解甚少。
分析与健康对照相比，MS患者循环CD8 T细胞的基因表达谱。
对纯化的CD8 T细胞的RNA进行测序，并分析差异基因表达。对几个p值临界值下的基因进行通路分析，以确定相关的假定通路。
我们在MS患者中鉴定出36个具有显著差异基因表达的基因。四个基因的表达差异至少达到2倍。大多数差异表达基因在MS患者中表达较高。全基因组关联研究（GWAS）中与MS相关的基因在差异表达基因中显示出富集。我们未在MS患者差异表达基因中鉴定出特定通路的富集。
MS患者的CD8 T细胞表现出差异基因表达，MS患者中基因活性主要更高。我们在研究中未鉴定出特定的生物学通路。对CD8 T细胞及其亚型进行更详细的分析可能会增进对T细胞如何参与MS的理解。
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