Department of Preventative Ophthalmology, Shanghai Eye Disease Prevention and Treatment Center, Shanghai, China.
Department of Ophthalmology, Fengcheng Hospital, Fengxian District, Shanghai, China.
Transl Vis Sci Technol. 2020 Dec 4;9(13):8. doi: 10.1167/tvst.9.13.8. eCollection 2020 Dec.
Diabetics are more prone to suffer from dry eye (DE). The ages of diabetes are decreasing, so ocular surface status in younger generations is worthy of attention. We used tandem mass tag (TMT)-labeled proteomics and weighted correlation network analysis (WGCNA) to identify differentially expressed proteins in the tear proteome of adults and children with diabetic DE.
Study subjects were divided into six groups of 10, including three groups each for adults and children. The adult groups included diabetics with DE (A), diabetics without DE (B), and normal controls (C); the corresponding groups of children were identified as (D), (E), and (F). DE tests were performed on all subjects. We extracted total proteins and labeled them with TMTs for analysis. WGCNA was used to recognize hub genes.
Tear film function was poorer in patients with diabetic DE. In adults, 1922 proteins were identified, and WGCNA analysis revealed three hub genes related to diabetic DE. For children, 2709 proteins were identified, and WGCNA analysis identified one hub gene related to diabetic DE. Kyoto Encyclopedia of Genes and Genomes analysis found similarities among metabolic pathways involved in differential expression of proteins in adult and child tear samples.
The pathogenesis of diabetic DE was highly similar in adults and children. The differentially expressed tear proteins in type 2 diabetes of adults and children was associated with inflammation, immune factors, and lipid metabolism.
Our findings found high similarities in the pathogenesis of diabetic DE in adults and children.
糖尿病患者更容易患有干眼症（DE）。糖尿病的发病年龄呈下降趋势，因此年轻一代的眼表状况值得关注。我们使用串联质量标签（TMT）标记蛋白质组学和加权相关网络分析（WGCNA）来鉴定成人和儿童糖尿病 DE 患者的泪液蛋白质组中的差异表达蛋白。
研究对象分为六组，每组 10 人，包括成人组和儿童组各三组。成人组包括糖尿病 DE 患者（A）、无糖尿病 DE 的糖尿病患者（B）和正常对照组（C）；相应的儿童组分别为（D）、（E）和（F）。所有受试者均进行 DE 测试。我们提取总蛋白并使用 TMT 进行标记以进行分析。使用 WGCNA 识别枢纽基因。
糖尿病 DE 患者的泪膜功能较差。在成人中，鉴定出 1922 种蛋白质，WGCNA 分析显示与糖尿病 DE 相关的三个枢纽基因。对于儿童，鉴定出 2709 种蛋白质，WGCNA 分析鉴定出与糖尿病 DE 相关的一个枢纽基因。京都基因与基因组百科全书分析发现，成人和儿童泪样中差异表达蛋白涉及的代谢途径存在相似性。
成人和儿童的糖尿病 DE 发病机制高度相似。成人和儿童 2 型糖尿病的差异表达泪蛋白与炎症、免疫因子和脂质代谢有关。
我们的研究结果发现，成人和儿童糖尿病 DE 的发病机制具有高度相似性。
