Liu Yongbo, Fu Cheng, Ye Shaotang, Liang Yingxin, Qi Zhonghe, Yao Congwen, Wang Zhen, Wang Ji, Cai Siqi, Tang Shiyu, Chen Ying, Li Shoujun
College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China.
Front Vet Sci. 2020 Dec 3;7:585071. doi: 10.3389/fvets.2020.585071. eCollection 2020.
Avian-origin H3N2 canine influenza viruses (CIVs) cause severe contagious respiratory disease in dogs, and quickly adapt to new environments. To further understand the mechanism of virus infection and host-virus interactions, we characterized the complete phosphoproteome of dogs infected with H3N2 CIV. Nine-week-old Beagle dogs were inoculated intranasally with 10 EID of A/canine/Guangdong/04/2014 (H3N2) virus. Lung sections were harvested at 5 days post-inoculation (dpi) and processed for global and quantitative analysis of differentially expressed phosphoproteins. A total of 1,235 differentially expressed phosphorylated proteins were identified in the dog lung after H3N2 CIV infection, and 3,016 modification sites were identified among all differentially expressed proteins. We then performed an enrichment analysis of functional annotations using Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) database analyses to predict the functions of the identified differential phosphoproteins. Our data indicate that H3N2 CIV infection causes dramatic changes in the host protein phosphorylation of dog lungs. To our knowledge, this is the first study to assess the effect of H3N2 CIV infection on the phosphoproteome of beagles. These data provide novel insights into H3N2-CIV-triggered regulatory phosphorylation circuits and signaling networks and may improve our understanding of the mechanisms underlying CIV pathogenesis in dogs.
禽源H3N2犬流感病毒（CIV）可在犬类中引发严重的传染性呼吸道疾病，并能迅速适应新环境。为了进一步了解病毒感染机制以及宿主与病毒的相互作用，我们对感染H3N2 CIV的犬类的完整磷酸化蛋白质组进行了表征。9周龄的比格犬经鼻接种10个鸡胚感染剂量（EID）的A/犬/广东/04/2014（H3N2）病毒。在接种后5天（dpi）采集肺组织切片，用于对差异表达的磷酸化蛋白质进行整体和定量分析。H3N2 CIV感染后，在犬肺中总共鉴定出1235种差异表达的磷酸化蛋白质，在所有差异表达的蛋白质中鉴定出3016个修饰位点。然后，我们使用京都基因与基因组百科全书（KEGG）和基因本体论（GO）数据库分析对功能注释进行富集分析，以预测所鉴定的差异磷酸化蛋白质的功能。我们的数据表明，H3N2 CIV感染会导致犬肺宿主蛋白磷酸化发生显著变化。据我们所知，这是第一项评估H3N2 CIV感染对比格犬磷酸化蛋白质组影响的研究。这些数据为H3N2 - CIV触发的调节磷酸化回路和信号网络提供了新的见解，并可能增进我们对犬类CIV发病机制的理解。
