College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou, China.
Front Cell Infect Microbiol. 2019 Mar 26;9:76. doi: 10.3389/fcimb.2019.00076. eCollection 2019.
This study aimed to detect changes in the complete transcriptome of MDCK cells after infection with the H5N1 and H3N2 canine influenza viruses using high-throughput sequencing, search for differentially expressed RNAs in the transcriptome of MDCK cells infected with H5N1 and H3N2 using comparative analysis, and explain the differences in the pathogenicity of H5N1 and H3N2 at the transcriptome level. Based on the results of our comparative analysis, significantly different levels of expression were found for 2,464 mRNAs, 16 miRNAs, 181 lncRNAs, and 262 circRNAs in the H3N2 infection group and 448 mRNAs, 12 miRNAs, 77 lncRNAs, and 189 circRNAs in the H5N1 infection group. Potential functions were predicted by performing Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of the target genes of miRNAs, lncRNAs and circRNAs, and the ncRNA-mRNA regulatory network was constructed based on differentially expressed RNAs. A greater number of pathways regulating immune metabolism were altered in the H3N2 infection group than in the H5N1 infection group, which may be one reason why the H3N2 virus is less pathogenic than is the H5N1 virus. This study provides detailed data on the production of ncRNAs during infection of MDCK cells by the canine influenza viruses H3N2 and H5N1, analyzed differences in the total transcriptomes between H3N2- and H5N1-infected MDCK cells, and explained these differences with regard to the pathogenicity of H3N2 and H5N1 at the transcriptional level.
本研究旨在通过高通量测序检测 H5N1 和 H3N2 犬流感病毒感染 MDCK 细胞后全转录组的变化，通过比较分析寻找 H5N1 和 H3N2 感染 MDCK 细胞中转录组中差异表达的 RNA，并从转录组水平解释 H5N1 和 H3N2 致病性的差异。基于我们的比较分析结果，在 H3N2 感染组中发现 2464 个 mRNAs、16 个 miRNAs、181 个 lncRNAs 和 262 个 circRNAs 的表达水平存在显著差异，在 H5N1 感染组中发现 448 个 mRNAs、12 个 miRNAs、77 个 lncRNAs 和 189 个 circRNAs 的表达水平存在显著差异。通过对 miRNA、lncRNA 和 circRNA 的靶基因进行基因本体（GO）和京都基因与基因组百科全书（KEGG）分析，预测了它们的潜在功能，并基于差异表达的 RNA 构建了 ncRNA-mRNA 调控网络。在 H3N2 感染组中，调节免疫代谢的通路比 H5N1 感染组发生了更多的改变，这可能是 H3N2 病毒比 H5N1 病毒致病性低的原因之一。本研究提供了犬流感病毒 H3N2 和 H5N1 感染 MDCK 细胞时 ncRNA 产生的详细数据，分析了 H3N2 和 H5N1 感染的 MDCK 细胞之间总转录组的差异，并从转录水平解释了 H3N2 和 H5N1 致病性的这些差异。
