Dipartimento di Malattie Infettive, Istituto Superiore di Sanità, Rome, Italy.
Dipartimento di Malattie Infettive, Istituto Superiore di Sanità, Rome, Italy - at present: Direzione Generale della Prevenzione Sanitaria, Ministero della Salute, Rome, Italy.
Ann Ist Super Sanita. 2020 Oct-Dec;56(4):430-436. doi: 10.4415/ANN_20_04_04.
Hyperinvasive strains of Neisseria meningitidis serogroup C have caused outbreaks of severe disease in Italy. Here, we report the analysis of the migration patterns of C:P1.5-1,10-8:F3-6:ST-11(cc11) meningococcal strains from different Italian regions collected between 2012 and 2017.
N. meningitidis genomes were sequenced through the whole genome sequencing (WGS) method and were analyzed using the BIGSdb Genome Comparator tool. The phylogeography was performed using BEAST. The gene flows in Italy were tested by using MacClade.
The C:P1.5-1,10-8:F3-6:ST-11(cc11) hyperinvasive meningococcal strain, for the data available at the time of the analysis, from UK reached at first Emilia Romagna region, and then, in 2012, was detected in the outbreak occurred in the port of Livorno. The "Tuscany-outbreak strain" was likely introduced in Italy between 2013 and 2014. Most of the observed gene flow events occurred from the Center to Northern part of Italy.
The phylogeographic analysis allowed to track the dissemination of C:P1.5-1,10-8:F3-6:ST-11(cc11) strains in the country.
侵袭性较强的 C 群脑膜炎奈瑟菌已在意大利引发了严重疾病的爆发。在此，我们报告了对 2012 年至 2017 年间收集的来自意大利不同地区的 C:P1.5-1,10-8:F3-6:ST-11(cc11)脑膜炎奈瑟菌株的迁移模式的分析。
通过全基因组测序 (WGS) 方法对脑膜炎奈瑟菌基因组进行测序，并使用 BIGSdb 基因组比较器工具进行分析。采用 BEAST 进行系统发育地理学分析。使用 MacClade 测试意大利的基因流。
在所分析的时间内，从英国到达艾米利亚-罗马涅地区的 C:P1.5-1,10-8:F3-6:ST-11(cc11) 侵袭性脑膜炎奈瑟菌株首先被发现，然后在 2012 年，在利沃诺港爆发的疫情中被检测到。“托斯卡纳爆发菌株”可能是在 2013 年至 2014 年期间引入意大利的。观察到的大多数基因流事件发生在意大利的中心到北部地区。
系统发育地理学分析可追踪 C:P1.5-1,10-8:F3-6:ST-11(cc11)菌株在该国的传播。
