一种用于超灵敏 Hg 检测的新型电化学生物传感器——三重信号放大策略。

A novel electrochemical biosensor for ultrasensitive Hg detection a triple signal amplification strategy.

机构信息

State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, China.

School of Tropical Medicine and Laboratory Medicine, Key Laboratory of Emergency and Trauma of Ministry of Education, Research Unit of Island Emergency Medicine, Chinese Academy of Medical Sciences (No. 2019RU013), Hainan Medical University, Haikou, 571199, China.

出版信息

Chem Commun (Camb). 2021 Jan 21;57(5):619-622. doi: 10.1039/d0cc07268k.

DOI:10.1039/d0cc07268k

PMID:33346300

Abstract

We developed a novel electrochemical biosensor for ultrasensitive Hg2+ detection via a triple signal amplification strategy of a DNA dual cycle, organic-inorganic hybrid nanoflowers (Cu3(PO4)2 HNFs) and gold nanoparticle (AuNP) probe. The DNA dual cycle was triggered by exonuclease III (Exo III) in the presence of Hg2+, and Cu3(PO4)2 HNFs were synthesized as an AuNP probe carrier. The electrochemical biosensor displayed high stability, high sensitivity and excellent specificity, which was improved by up to seven orders of magnitude compared to the World Health Organization (WHO) allowed Hg2+ levels in drinking water. This signal amplification strategy could be easily modified and extended to detect other hazardous heavy metals and nucleic acids.

摘要

我们开发了一种新型电化学生物传感器，通过 DNA 双循环、有机-无机杂化纳米花（Cu3(PO4)2 HNFs）和金纳米粒子（AuNP）探针的三重信号放大策略，实现对 Hg2+的超灵敏检测。在存在 Hg2+的情况下，DNA 双循环被外切酶 III（Exo III）触发，并且 Cu3(PO4)2 HNFs 被合成作为 AuNP 探针载体。与世界卫生组织（WHO）允许的饮用水中 Hg2+水平相比，电化学生物传感器显示出高稳定性、高灵敏度和优异的特异性，灵敏度提高了多达七个数量级。这种信号放大策略可以很容易地修改和扩展，以检测其他危险的重金属和核酸。