Collaborative Innovation Center for Modern Grain Circulation and Safety, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, Jiangsu, China.
Collaborative Innovation Center of Grain Storage Security, Zhengzhou, Henan, China.
J Econ Entomol. 2021 Feb 9;114(1):440-453. doi: 10.1093/jee/toaa273.
The rusty grain beetle, Cryptolestes ferrugineus (Stephens), is a serious pest of stored grain, which has developed high levels of resistance to phosphine. In this study, five geographically distant populations of C. ferrugineus had been collected in China, specifically in granaries where phosphine fumigant is used for pest control, and they showed a high resistance ratio up to 1,907 (LC50 = 21.0 mg/liter). Then, a reference transcriptome was constructed to use as a basis for investigating the molecular mechanisms of phosphine resistance in this species, which consisted of 47,006 unigenes with a mean length of 1,090. Subsequently, the RNA-Seq analysis of individuals from the most susceptible and resistant populations led to the identification of 54 genes that are differentially expressed. GO and KEGG analysis demonstrated that genes associated with mitochondrial and respiration functions were significantly enriched. Also, the 'structural constituent of cuticle' term was annotated in the GO enrichment analysis and further qRT-PCR confirmed that the expression levels of nine cuticular protein genes were significantly increased in the resistant population. In conclusion, we present here a transcriptome-wide overview of gene expression changes between resistant and susceptible populations of C. ferrugineus, and this in turn documents that mitochondria and cuticular protein genes may play together a crucial role in phosphine resistance. Further gene function analysis should enable the provision of advice to expedite resistance management decisions.
锈赤扁谷盗（Cryptolestes ferrugineus （Stephens））是一种严重的储粮害虫，对磷化氢已产生高水平抗性。在本研究中，收集了来自中国的五个地理上遥远的锈赤扁谷盗种群，这些种群均来自使用磷化氢熏蒸剂进行害虫防治的粮仓，其抗性比高达 1907（LC50=21.0 毫克/升）。然后，构建了参考转录组，作为研究该物种磷化氢抗性分子机制的基础，该转录组由 47006 个平均长度为 1090 的 unigenes组成。随后，对最敏感和最具抗性的种群个体进行 RNA-Seq 分析，鉴定出 54 个差异表达的基因。GO 和 KEGG 分析表明，与线粒体和呼吸功能相关的基因显著富集。此外，GO 富集分析中注释了“表皮结构成分”这一术语，进一步的 qRT-PCR 验证表明，在抗性种群中，九个表皮蛋白基因的表达水平显著增加。总之，我们在此呈现了锈赤扁谷盗抗性和敏感种群之间全基因组表达变化的概述，这表明线粒体和表皮蛋白基因可能共同在磷化氢抗性中发挥关键作用。进一步的基因功能分析将有助于为加快抗性管理决策提供建议。
