Tian Yuan-Yuan, Li Bo-Nan, Zhou Hai-Liang, Zhou Qing, Zhou Xing, Xing Jun-Ping
Department of Urology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
Department of Andrology, The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410000, China.
Zhonghua Nan Ke Xue. 2020 Mar;26(3):237-241.
To investigate the effect of Jiarong Tablets (JRT) on the testicular morphology and function of rats with late-onset hypogonadism (LOH).
LOH models were established in 8 eighteen-month-old male SD rats, treated intragastrically with distilled water (the model control group, n = 4) or JRT at 0.375 g/kg/d, qd (the JRT group, n = 4), and another 5 two-month-old normal male SD rats were also given distilled water by gavage (normal control group), all for 28 days. Then all the rats were weighed and sacrificed for measurement of the serum T level and pathological and electron microscopic examination of the testis tissue.
Compared with the normal controls, the LOH models showed significantly decreased testis coefficient (P < 0.05) and serum T level (［3.40 ± 0.06］ vs ［5.88 ± 0.46］ ng /ml, P < 0.05). No statistically significant differences were observed in the model control and JRT groups in the body weight and testis coefficient (P > 0.05), but the serum T level (［4.50 ± 0.78］ ng/ml) was remarkably decreased in the latter (P < 0.05). In comparison with the model controls, the rats treated with JRT exhibited increases in the sperm count in the seminiferous tubules and the amount of testicular interstitial cells. Electron microscopy revealed a markedly increased number of mitochondria in the JRT-treated animals, with some mitochondrial sheaths and cristae but no obvious mitochondrial edema.
Jiarong Tablets can elevate the serum T level and improve the testicular morphology and ultrastructure of LOH rats.
探讨佳蓉片（JRT）对迟发性性腺功能减退（LOH）大鼠睾丸形态及功能的影响。
将8只18月龄雄性SD大鼠建立LOH模型，分别灌胃蒸馏水（模型对照组，n = 4）或0.375 g/kg/d的佳蓉片（JRT组，n = 4），qd，共28天。另取5只2月龄正常雄性SD大鼠灌胃蒸馏水作为正常对照组。之后称取所有大鼠体重并处死，检测血清睾酮（T）水平，对睾丸组织进行病理及电镜检查。
与正常对照组相比，LOH模型大鼠睾丸系数及血清T水平显著降低（P < 0.05）（睾丸系数：［3.40 ± 0.06］ vs ［5.88 ± 0.46］，血清T水平：［3.40 ± 0.06］ vs ［5.88 ± 0.46］ ng/ml，P < 0.05）。模型对照组与JRT组大鼠体重及睾丸系数比较，差异无统计学意义（P > 0.05），但JRT组血清T水平显著降低（［4.50 ± 0.78］ ng/ml，P < 0.05）。与模型对照组相比，JRT治疗的大鼠生精小管内精子数量及睾丸间质细胞数量增加。电镜检查显示，JRT治疗的动物线粒体数量明显增加，有一些线粒体膜和嵴，但无明显线粒体水肿。
佳蓉片可提高LOH大鼠血清T水平，改善其睾丸形态及超微结构。
