Department of Biological and Medical Sciences, Faculty of Health and Life Sciences, Oxford Brookes University, Oxford, UK.
Laboratoire « Signalisation Fonctionnelle des Canaux Ioniques et Récepteurs » (SiFCIR), UPRES-EA2647 USC INRAE 1330, SFR 4207 QUASAV, UFR Sciences, Université d'Angers, Angers, France.
Pest Manag Sci. 2021 Aug;77(8):3787-3799. doi: 10.1002/ps.6245. Epub 2021 Jan 19.
Cockroaches are serious urban pests that can transfer disease-causing microorganisms as well as trigger allergic reactions and asthma. They are commonly managed by pesticides that act on cys-loop ligand-gated ion channels (cysLGIC). To provide further information that will enhance our understanding of how insecticides act on their molecular targets in cockroaches, we used genome and reverse transcriptase polymerase chain reaction (RT-PCR) data to characterize the cysLGIC gene superfamilies from Blattella germanica and Periplaneta americana.
The B. germanica and P. americana cysLGIC superfamilies consist of 30 and 32 subunit-encoding genes, respectively, which are the largest insect cysLGIC superfamilies characterized to date. As with other insects, the cockroaches possess ion channels predicted to be gated by acetylcholine, γ-aminobutyric acid, glutamate and histamine, as well as orthologues of the drosophila pH-sensitive chloride channel (pHCl), CG8916 and CG12344. The large cysLGIC superfamilies of cockroaches are a result of an expanded number of divergent nicotinic acetylcholine receptor subunits, with B. germanica and P. americana, respectively, possessing eight and ten subunit genes. Diversity of the cockroach cysLGICs is also broadened by alternative splicing and RNA A-to-I editing. Unusually, both cockroach species possess a second glutamate-gated chloride channel as well as another CG8916 subunit.
These findings on B. germanica and P. americana enhance our understanding of the evolution of the insect cysLGIC superfamily and provide a useful basis for the study of their function, the detection and management of insecticide resistance, and for the development of improved pesticides with greater specificity towards these major pests. © 2020 Society of Chemical Industry.
蟑螂是严重的城市害虫，它们可以传播致病微生物，还会引发过敏反应和哮喘。它们通常通过作用于 cys 环配体门控离子通道（cysLGIC）的杀虫剂来进行治理。为了提供更多信息，以增强我们对杀虫剂在蟑螂分子靶标上作用方式的理解，我们使用基因组和逆转录聚合酶链反应（RT-PCR）数据来对德国小蠊和美洲大蠊的 cysLGIC 基因超家族进行了特征描述。
B. germanica 和 P. americana 的 cysLGIC 超家族分别由 30 和 32 个亚基编码基因组成，这是迄今为止特征描述的最大昆虫 cysLGIC 超家族。与其他昆虫一样，蟑螂拥有预测由乙酰胆碱、γ-氨基丁酸、谷氨酸和组氨酸门控的离子通道，以及果蝇 pH 敏感氯离子通道（pHCl）、CG8916 和 CG12344 的同源物。蟑螂 cysLGIC 超大家族的数量庞大，是由于烟碱型乙酰胆碱受体亚基的数量不断增加，B. germanica 和 P. americana 分别拥有 8 个和 10 个亚基基因。蟑螂 cysLGIC 的多样性也通过选择性剪接和 RNA A 到 I 的编辑得到了扩展。不寻常的是，这两种蟑螂都拥有第二种谷氨酸门控氯离子通道以及另一个 CG8916 亚基。
这些关于 B. germanica 和 P. americana 的发现增强了我们对昆虫 cysLGIC 超家族进化的理解，并为研究其功能、检测和管理杀虫剂抗性以及开发针对这些主要害虫的特异性更强的改良杀虫剂提供了有用的基础。© 2020 化学工业协会。
