Department of Invertebrate Zoology, Faculty of Biology, Saint Petersburg State University, 199034 Universitetskaya nab. 7/9, Saint Petersburg, Russia; Laboratory of Cytology of Unicellular Organisms, Institute of Cytology RAS, Tikhoretsky ave. 4, St. Petersburg 194064, Russia.
Department of Invertebrate Zoology, Faculty of Biology, Saint Petersburg State University, 199034 Universitetskaya nab. 7/9, Saint Petersburg, Russia.
Eur J Protistol. 2021 Feb;77:125759. doi: 10.1016/j.ejop.2020.125759. Epub 2020 Nov 19.
A new species of the "proteus-type" naked amoebae (large cells with discrete tubular pseudopodia) was isolated from tree bark sample of a birch tree in the surrounding of Kislovodsk town, Russia and named Polychaos centronucleolus n. sp. (Amoebozoa, Tubulinea). Amoebae of this species have a filamentous cell coat and a nucleus with a central compact nucleolus. This type of nucleolar organization has not been previously known for the genus Polychaos. A sequence of the 18S rRNA gene of this strain was obtained using whole genome amplification of DNA from the single amoeba cell, followed by NGS sequencing. The analysis of molecular data robustly groups this species with Polychaos annulatum within the family Hartmannellidae. Our results, together with the results of our previous studies, show that the taxonomic assignment of "proteus-type" amoebae species is becoming increasingly complex, and the taxonomic characters that can be used to classify these organisms are becoming more shadowed.
一种新型的“变形虫型”裸质变形虫（具有离散管状伪足的大细胞）从俄罗斯基斯洛沃茨克镇周围的桦树皮样本中分离出来，并被命名为 Polychaos centronucleolus n. sp.（变形虫门，管状纲）。该物种的变形虫具有丝状细胞外套和带有中央致密核仁的核。这种核仁组织类型以前在 Polychaos 属中尚未被发现。使用从单个变形虫细胞中扩增的 DNA 进行全基因组扩增，获得了该菌株的 18S rRNA 基因序列，随后进行了 NGS 测序。分子数据分析的结果强烈将该物种与 Hartmannellidae 科的 Polychaos annulatum 归为一组。我们的研究结果与之前的研究结果一起表明，“变形虫型”变形虫物种的分类分配变得越来越复杂，可用于对这些生物进行分类的分类特征变得更加模糊。
