利用光漂白后线性荧光恢复定义模型膜中的扩散。

Defining the Diffusion in Model Membranes Using Line Fluorescence Recovery after Photobleaching.

作者信息

Kure Jakob L, Andersen Camilla B, Rasmussen Thomas E, Lagerholm B Christoffer, Arnspang Eva C

机构信息

SDU Biotechnology, Department of Green Technology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.

MEMPHYS, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark.

出版信息

Membranes (Basel). 2020 Dec 17;10(12):434. doi: 10.3390/membranes10120434.

DOI:10.3390/membranes10120434

PMID:33348780

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7767200/

Abstract

In this study, we explore the use of line FRAP to detect diffusion in synthetic lipid membranes. The study of the dynamics of these membrane lipids can, however, be challenging. The diffusion in two different synthetic membranes consisting of the lipid mixtures 1:1 DOPC:DPPC and 2:2:1 DOPC:DPPC:Cholesterol was studied with line FRAP. A correlation between diffusion coefficient and temperature was found to be dependent on the morphology of the membrane. We suggest line FRAP as a promising accessible and simple technique to study diffusion in plasma membranes.

摘要

在本研究中，我们探索使用线性荧光漂白恢复技术（line FRAP）来检测合成脂质膜中的扩散。然而，对这些膜脂动力学的研究可能具有挑战性。我们使用线性荧光漂白恢复技术研究了由1:1二油酰磷脂酰胆碱（DOPC）:二棕榈酰磷脂酰胆碱（DPPC）和2:2:1二油酰磷脂酰胆碱:二棕榈酰磷脂酰胆碱:胆固醇组成的两种不同合成膜中的扩散。发现扩散系数与温度之间的相关性取决于膜的形态。我们认为线性荧光漂白恢复技术是一种有前景的、易于使用且简单的技术，可用于研究质膜中的扩散。