Department of Urology, University of Health Sciences, Trabzon Kanuni Training and Research Hospital.
Marmara University School of Medicine, Department of Urology, Istanbul.
Arch Ital Urol Androl. 2020 Dec 18;92(4). doi: 10.4081/aiua.2020.4.340.
To date, various molecules have been investigated to reduce the effect of renal ischemia/reperfusion (I/R) injury. However, none have yet led to clinical use. The present study aimed to investigate the protective effect of cordycepin (C) on renal I/R injury in an experimental rat model.
Twenty-four mature Sprague Dawley female rat was randomly divided into three groups: Sham, I/R, I/R+C. All animals underwent abdominal exploration. To induce I/R injury, an atraumatic vascular bulldog clamp was applied to the right renal pedicle for 60 minutes (ischemia) and later clamp was removed to allow reperfusion in all rats, except for the sham group. In the I/R + C group, 10 mg/kg C was administered intraperitoneally, immediately after reperfusion. After 4 hours of reperfusion, the experiment was terminated with right nephrectomy. Histological studies and biochemical analyses were performed on the right nephrectomy specimens. EGTI (endothelial, glomerular, tubulointerstitial) histopathology scoring and semi-quantitative analysis of renal cortical necrosis were used for histological analyses and superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), total oxidant status (TOS) for biochemical analyses.
Histopathological examination of the tissue damage revealed that all kidneys in the sham group were normal. The I/R group had higher histopathological scores than the I/R + C group. In the biochemical analysis of the tissues, SOD, MDA, TOS values were found to be statistically different in the I/R group compared to the I/R + C group (p: 0.004, 0.004, 0.001 respectively).
Intraperitoneal cordycepin injection following ischemia preserve renal tissue against oxidative stress in a rat model of renal I/R injury.
迄今为止，已经有多种分子被研究用于减轻肾缺血再灌注（I/R）损伤的影响，但没有一种分子最终应用于临床。本研究旨在探讨蛹虫草素（C）对实验性大鼠肾 I/R 损伤的保护作用。
24 只成熟的 Sprague Dawley 雌性大鼠随机分为三组：假手术组、I/R 组、I/R+C 组。所有动物均行腹部探查。为诱导 I/R 损伤，在所有大鼠的右肾蒂上应用无创伤性血管夹夹闭 60 分钟（缺血），除假手术组外，所有大鼠均松开夹闭以允许再灌注。在 I/R+C 组中，在再灌注后立即给予 10mg/kg C 腹膜内注射。再灌注 4 小时后，行右肾切除术终止实验。对右肾切除标本进行组织学研究和生化分析。采用 EGTI（内皮、肾小球、小管间质）组织病理学评分和肾皮质坏死的半定量分析进行组织学分析，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）、总氧化状态（TOS）进行生化分析。
组织损伤的组织学检查显示，假手术组的所有肾脏均正常。I/R 组的组织病理学评分高于 I/R+C 组。组织生化分析中，I/R 组的 SOD、MDA、TOS 值与 I/R+C 组相比差异有统计学意义（p：0.004、0.004、0.001）。
在大鼠肾 I/R 损伤模型中，缺血后腹膜内注射蛹虫草素可防止肾组织发生氧化应激。
