Department of Urology, Aydin State Hospital, Aydın-Turkey.
Department of Urology, Suleyman Demirel University Faculty of Medicine, Isparta-Turkey.
Ulus Travma Acil Cerrahi Derg. 2022 Mar;28(3):344-351. doi: 10.14744/tjtes.2021.15740.
Background: Renal ischemia-reperfusion injury (RIRI) is the most frequent cause of acute renal failure in clinical conditions such as trauma and shock as well as renal surgeries. Oxerutin is a member of the flavonoid family and possesses antioxidant properties. The aim of this study was to investigate whether oxerutin has protective effects on RIRI.
Twenty-eight male Wistar albino rats were randomly divided into three groups: sham control group (n=8), RIRI group (n=10), and RIRI + oxerutin group (n=10). RIRI was achieved by clamping the left renal artery for 30 min, followed 1-h reperfusion period. Thereafter, blood samples and left kidney tissue samples were taken for histopathological and biochemical examination. Blood urea nitrogen (BUN), urea, creatinine, and cystatin C levels, which are indicators of kidney function, as well as tumor necrosis factor-alpha, which is an indicator of inflammation were analyzed in blood samples. Total antioxidant status and total oxidant status (TOS), which are indicators of oxidative stress were analyzed on renal tissues. The apoptotic index, an indicator of kidney damage, as well as histopathological changes were evaluated on renal tissues.
The apoptotic index, TOS, tumor necrosis factor-alpha, BUN, and urea levels were lower in the RIRI + oxerutin group than in the RIRI group (p<0.05). The results demonstrated that the histopathological and biochemical properties of oxerutin protected rats from RIRI.
The findings obtained in this study show that prophylactic administration of oxerutin has protective effects on apoptosis and renal failure caused by RIRI. Therefore, oxerutin can be used as an effective prophylactic agent in the treatment of RIRI.
背景：肾缺血再灌注损伤（RIRI）是创伤和休克以及肾手术等临床情况下急性肾衰竭的最常见原因。芦丁是黄酮类家族的一员，具有抗氧化特性。本研究旨在探讨芦丁是否对 RIRI 具有保护作用。
28 只雄性 Wistar 白化大鼠随机分为三组：假手术对照组（n=8）、RIRI 组（n=10）和 RIRI+芦丁组（n=10）。通过夹闭左肾动脉 30 分钟，再灌注 1 小时来实现 RIRI。此后，采集血样和左肾组织样本进行组织病理学和生化检查。分析血样中肾功能指标血尿素氮（BUN）、尿素、肌酐和胱抑素 C 水平以及炎症指标肿瘤坏死因子-α。分析肾组织中氧化应激指标总抗氧化状态和总氧化态（TOS）。评估肾组织中的细胞凋亡指数、肾功能损伤指标以及组织病理学变化。
与 RIRI 组相比，RIRI+芦丁组的细胞凋亡指数、TOS、肿瘤坏死因子-α、BUN 和尿素水平较低（p<0.05）。结果表明，芦丁对 RIRI 大鼠具有保护作用。
本研究结果表明，芦丁预防性给药对 RIRI 引起的细胞凋亡和肾衰竭具有保护作用。因此，芦丁可作为治疗 RIRI 的有效预防剂。
