Department of Dental Materials and Prosthodontics, São Paulo State University (Unesp), School of Dentistry, Araraquara, São Paulo, Brazil.
School of Dentistry, University Santo Tomas, Bucaramanga, Colombia.
Biofouling. 2020 Nov;36(10):1196-1209. doi: 10.1080/08927014.2020.1862093. Epub 2020 Dec 21.
Biofilms were developed from human saliva on bovine enamel discs in four experimental conditions to investigate dental caries development: feast and famine (M1), abundance and scarcity (M2), three meals daily (M3), and three meals plus two snacks daily (M4). The main difference between these models was the diet for microbial growth. The evaluations included verifying the pH of the spent culture media and analyzing the enamel discs for demineralization (microhardness and roughness) and biofilms (biomass, viable populations of mutans streptococci, and total microbiota). Two major behaviors were observed: M1 and M2 promoted an acidic environment, while M3 and M4 maintained pH values closer to neutral. The demineralization process was slower in the neutral groups but more pronounced in M3, while a greater increase in microbiota and biomass was observed over time for both neutral groups. Thus, the M3 model was better at mimicking the oral environment that leads to demineralization.
在四种实验条件下，用人唾液在牛牙釉质圆盘上形成生物膜，以研究龋齿的发展：饱食与饥饿（M1）、丰富与匮乏（M2）、一日三餐（M3）和一日三餐加两份零食（M4）。这些模型的主要区别在于微生物生长的饮食。评估包括验证消耗的培养介质的 pH 值，并分析牙釉质圆盘的脱矿质（显微硬度和粗糙度）和生物膜（生物量、变形链球菌的活菌数和总微生物群）。观察到两种主要行为：M1 和 M2 促进酸性环境，而 M3 和 M4 保持 pH 值更接近中性。中性组的脱矿过程较慢，但在 M3 中更为明显，而两组的微生物群和生物量随着时间的推移都有较大的增加。因此，M3 模型更能模拟导致脱矿质的口腔环境。
