Hsieh L L, Peraino C, Weinstein I B
Cancer Center, Columbia University, New York, New York 10032.
Cancer Res. 1988 Jan 15;48(2):265-9.
The expression of two cellular oncogenes (c-myc and c-Ha-ras), the epidermal growth factor receptor gene, and two endogenous retrovirus-like sequences (rat leukemia virus and 30S) was examined in control (nonregenerating) rat livers and at various times after partial hepatectomy. One group of rats had been fed phenobarbital (0.05%) for 16 days prior to the partial hepatectomy. The feeding of phenobarbital (0.05%) itself led to a 65% decrease in the level of epidermal growth factor receptor RNA, but no major change in the level of c-myc, H-ras, rat leukemia virus, or 30S RNAs, in the control rat livers. There was a considerable increase (4- to 5-fold) in the level of c-myc transcripts, at 12 and 48 h after partial hepatectomy in the phenobarbital-treated rats, and at 12 and 24 h in the rats on the control diet. By 72 h, the level of c-myc transcripts returned to normal in both groups of rats. A slight increase (about 1.5-fold) in the level of c-H-ras transcripts was seen at 24 h, which returned to normal levels by 168 h, in the regenerating livers of both the phenobarbital-treated and control diet rats. The regenerating livers displayed a marked decrease (3- to 4-fold) in the level of epidermal growth factor receptor RNA in both the phenobarbital and control diet rats. A marked increase (5- to 6-fold) in the level of transcripts homologous to the endogenous rat leukemia virus-like sequence was seen at 24 h in all of the regenerating livers, but there was no significant change in the level of RNAs homologous to 30S. Thus, the proliferation of normal rat liver cells mimics some but not all of the changes in mRNA levels that we have previously described in rat liver tumors.
在对照（未再生）大鼠肝脏以及部分肝切除术后的不同时间，检测了两种细胞癌基因（c-myc和c-Ha-ras）、表皮生长因子受体基因以及两种内源性逆转录病毒样序列（大鼠白血病病毒和30S）的表达。一组大鼠在部分肝切除术前已喂食苯巴比妥（0.05%）16天。在对照大鼠肝脏中，喂食苯巴比妥（0.05%）本身导致表皮生长因子受体RNA水平降低65%，但c-myc、H-ras、大鼠白血病病毒或30S RNA水平无重大变化。在苯巴比妥处理的大鼠中，部分肝切除术后12小时和48小时，以及对照饮食大鼠在12小时和24小时时，c-myc转录本水平有相当大的增加（4至5倍）。到72小时时，两组大鼠的c-myc转录本水平均恢复正常。在苯巴比妥处理和对照饮食大鼠的再生肝脏中，c-H-ras转录本水平在24小时时略有增加（约1.5倍），到168小时时恢复到正常水平。在苯巴比妥和对照饮食大鼠的再生肝脏中，表皮生长因子受体RNA水平均显著降低（3至4倍）。在所有再生肝脏中，与内源性大鼠白血病病毒样序列同源的转录本水平在24小时时显著增加（5至6倍），但与30S同源的RNA水平无显著变化。因此，正常大鼠肝细胞的增殖模拟了我们之前在大鼠肝肿瘤中描述的mRNA水平变化中的一些但不是全部变化。
