Lock A J, van Denderen J, Aarden L A
Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Endocrinology. 1988 Jan;122(1):319-24. doi: 10.1210/endo-122-1-319.
A monoclonal antibody directed against bovine TSH was obtained by hybridoma technology. This antibody was specific for TSH and did not react with bovine LH and FSH. Affinity chromatography of crude TSH was performed on anti-TSH Sepharose. Bovine TSH was purified in a single step to near homogeneity by this technique, as shown by cation exchange chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified TSH. The biological activity of the hormone was not affected during the purification, as determined by [3H]thymidine incorporation of the TSH-dependent FRTL5 cell line. The results indicate that affinity purification of TSH by means of a monoclonal antibody is a simple one-step procedure for the production of biologically active, highly purified TSH.
通过杂交瘤技术获得了一种针对牛促甲状腺激素（TSH）的单克隆抗体。该抗体对TSH具有特异性，不与牛促黄体生成素（LH）和促卵泡激素（FSH）发生反应。利用抗TSH琼脂糖进行粗制TSH的亲和层析。通过该技术，牛TSH一步纯化至接近均一性，纯化后的TSH经阳离子交换层析和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析表明了这一点。通过依赖TSH的FRTL5细胞系的[3H]胸苷掺入法测定，纯化过程中该激素的生物活性未受影响。结果表明，利用单克隆抗体亲和纯化TSH是一种生产具有生物活性的高纯度TSH的简单一步法。
