Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin, 301617, China.
Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, West Area, Tuanbo New Town, Jinghai District, Tianjin, 301617, China.
Neurosci Lett. 2021 Feb 16;746:135585. doi: 10.1016/j.neulet.2020.135585. Epub 2020 Dec 19.
Fluoxetine hydrochloride is one of the familiar antidepressants of the second generation and has the effect of inhibiting the reuptake of 5-hydroxytryptamine by central nervous system. Both clinical trials and animal experiments show that it has good antidepressant effect, but there are few reports on its clinical efficacy in treating depression patients from the perspective of metabolomics. This study aimed at evaluating the antidepressant effect of fluoxetine hydrochloride by metabolomics, so that to find out its specific biomarkers and related metabolic characteristics of depression in the treatment of depression and analyze the intervention mechanism of fluoxetine hydrochloride in depression.
Twenty depression patients and twenty healthy volunteers were recruited in clinical. Using ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) to analyze serum metabolites of depression patients pretherapy and post-treatment and compared with healthy people.
Finally, we have detected 16 specific biomarkers of depression. Compared with the healthy group, the level of 10 biomarkers in the depression group was significantly increased (P < 0.05) and 6 biomarkers were significantly decreased (P < 0.01). After 8 weeks of fluoxetine hydrochloride treatment, all the biomarkers have showed a tendency of callback. The metabolic pathways involved amino acid metabolism, energy metabolism and lipid metabolism.
In our study, the antidepressant effect of fluoxetine hydrochloride in clinic was proved by metabolomics and provided basis for clinical use of fluoxetine hydrochloride. At the same time, the biomarkers that may be related to the occurrence of depression are determined to provide objective basis for the diagnosis of depression.
盐酸氟西汀是第二代常见的抗抑郁药之一，具有抑制中枢神经系统 5-羟色胺再摄取的作用。临床试验和动物实验均表明其具有良好的抗抑郁作用，但从代谢组学角度观察其治疗抑郁症患者的临床疗效的报道较少。本研究旨在通过代谢组学评价盐酸氟西汀的抗抑郁作用，从而找出其治疗抑郁症的具体生物标志物及相关代谢特征，并分析盐酸氟西汀干预抑郁症的作用机制。
临床招募 20 例抑郁症患者和 20 例健康志愿者。采用超高效液相色谱-四极杆飞行时间质谱联用（UPLC-Q-TOF/MS）技术分析抑郁症患者治疗前和治疗后血清代谢物，并与健康人进行比较。
最终共检测到 16 种抑郁症的特异性生物标志物。与健康组相比，抑郁症组 10 种生物标志物水平显著升高（P<0.05），6 种生物标志物水平显著降低（P<0.01）。经过 8 周的盐酸氟西汀治疗后，所有的生物标志物均呈现回调的趋势。涉及的代谢途径有氨基酸代谢、能量代谢和脂质代谢。
本研究通过代谢组学证实了盐酸氟西汀在临床上的抗抑郁作用，为临床应用盐酸氟西汀提供了依据。同时确定了可能与抑郁症发生相关的生物标志物，为抑郁症的诊断提供了客观依据。
