Wu Zixuan, Liu Yujie, Zheng Jiaqi, Zhou Yongzhao, Xing Kefan, Sun Yuying, Zhang Jiquan
School of Life Sciences, Institute of Life Sciences and Green Development, Hebei University, Baoding, 071002, China.
School of Life Sciences, Institute of Life Sciences and Green Development, Hebei University, Baoding, 071002, China.
Fish Shellfish Immunol. 2021 Feb;109:82-86. doi: 10.1016/j.fsi.2020.12.009. Epub 2020 Dec 19.
Arginine kinase (AK, EC 2.7.3.3) plays an important role in cells with high, fluctuating energy requirements. In invertebrates, AK is the major phosphagen kinase that modulates the energy metabolism. Here, the full-length cDNA sequence encoding arginine kinase (EcAK) was obtained from the Exopalaemon carinicauda. The complete nucleotide sequence of EcAK contained a 1068 bp open reading frame (ORF) encoding EcAK precursor of 355 amino acids. The genomic DNA fragment of EcAK with the corresponding cDNA sequence is composed of 4 exons and 3 introns. The domain architecture of the deduced EcAK protein contained an ATP-gua_PtransN domain and an ATP-gua_Ptrans domain. EcAK mRNA was predominantly expressed in the muscle. The expression of EcAK in the prawns challenged with Vibrio parahaemolyticus and Aeromonas hydrophila changed in a time-dependent manner. Then, EcAK was recombinantly expressed in Pichia pastoris and the purified recombinant EcAK had the same enzymatic characterization as AK from the muscle of Euphausia superba. In conclusion, EcAK may play the same biological activity in E. carinicauda as those from other crustaceans.
精氨酸激酶（AK，EC 2.7.3.3）在能量需求高且波动的细胞中发挥着重要作用。在无脊椎动物中，AK是调节能量代谢的主要磷酸原激酶。在此，从脊尾白虾中获得了编码精氨酸激酶（EcAK）的全长cDNA序列。EcAK的完整核苷酸序列包含一个1068 bp的开放阅读框（ORF），编码355个氨基酸的EcAK前体。EcAK的基因组DNA片段与相应的cDNA序列由4个外显子和3个内含子组成。推导的EcAK蛋白的结构域架构包含一个ATP-gua_PtransN结构域和一个ATP-gua_Ptrans结构域。EcAK mRNA主要在肌肉中表达。用副溶血性弧菌和嗜水气单胞菌攻击的对虾中EcAK的表达呈时间依赖性变化。然后，EcAK在毕赤酵母中重组表达，纯化的重组EcAK具有与南极磷虾肌肉中的AK相同的酶学特性。总之，EcAK在脊尾白虾中可能发挥与其他甲壳类动物相同的生物学活性。
