Department of the Biology of Geological Processes, Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, 26111 Oldenburg, Germany.
Syst Appl Microbiol. 2021 Jan;44(1):126173. doi: 10.1016/j.syapm.2020.126173. Epub 2020 Dec 7.
Recent developments in fluorescence in situ hybridization (FISH) methods allow the detection and visualization of the genes/genomic regions of bacteria, archaea and infecting viruses at the single cell level. These methods use mixtures of polynucleotides as probes to specifically detect the target of interest. Gene-PROBER enables the design of polynucleotide mixtures for targeting genes or genomic regions in microorganisms. It has four workflows, depending on the availability of non-target sequences and the choice of probe synthesis, either by chemical synthesis or by PCR. It outputs polynucleotides that are spread along the target sequence and have similar melting properties. Therefore, such a polynucleotide mixture can be used as a single probe, in a single hybridization reaction. Gene-PROBER is a freely available web service that can be accessed at http://gene-prober.icbm.de/, and is implemented in the R language using the Shiny package.
荧光原位杂交 (FISH) 方法的最新进展使得能够在单细胞水平上检测和可视化细菌、古菌和感染病毒的基因/基因组区域。这些方法使用多核苷酸混合物作为探针来特异性地检测感兴趣的目标。Gene-PROBER 能够设计用于靶向微生物中基因或基因组区域的多核苷酸混合物。它有四个工作流程，具体取决于非目标序列的可用性和探针合成的选择，通过化学合成或 PCR 进行。它输出沿着目标序列分布且具有相似熔解特性的多核苷酸。因此，这样的多核苷酸混合物可以在单个杂交反应中用作单个探针。Gene-PROBER 是一个免费的网络服务，可以在 http://gene-prober.icbm.de/ 上访问，它是在 R 语言中使用 Shiny 包实现的。
