Australian Centre for Research on Separation Science, School of Chemistry, Monash University, Wellington Road, Clayton, VIC 3800, Australia.
Australian National Phenome Centre, Harry Perkins Institute of Medical Research, 5 Robin Warren Drive, Murdoch, Western Australia 6150, Australia.
J Chromatogr A. 2021 Jan 11;1636:461788. doi: 10.1016/j.chroma.2020.461788. Epub 2020 Dec 14.
In contrast to the well-known comprehensive two-dimensional gas chromatography (GC×GC) method, it is possible to define comprehensive multidimensional gas chromatography. 'Comprehensiveness' relates to analysis of the whole sample. Two-dimensional and multidimensional here refer to the use of at least two separation stages for analysis, however comprehensive 2DGC now appears to be reserved for the GC×GC method. This may be differentiated from comprehensive MDGC (CMDGC) simply by the analysis time assigned to the second (D) column, although there does not appear to be a specific definition that relates to this analysis time parameter. A number of different implementation protocols for comprehensive MDGC are described here, that may involve either a single, or multiple, injection(s). In all cases, independent retention must be achieved on each dimension to ensure the probability of enhanced separation. An original application of a crude oil sample is presented to illustrate development of the MDGC approach that incorporates two Deans switches (DS) and a cryogenic trapping approach, performed using a sequential heart-cut (H/C) event method incremented by 0.5 min for each injection; a total of 40 injections is used to analyse the total sample. The higher peak capacity and consequently greater resolution on the long D column is illustrated, compared with that expected for conventional GC×GC, with tentative identification in order to classify chemical classes. Incorporating an approach to acquiring retention indices may be implemented, although its utility for petroleum hydrocarbons is limited. Structured groupings of different chemical classes, as exemplified by mono and diaromatics for the crude oil sample, were noted.
与广为人知的全二维气相色谱（GC×GC）方法相比，现在可以定义全多维气相色谱。“全面性”与对整个样品的分析有关。二维和多维这里指的是至少使用两个分离阶段进行分析，然而综合二维气相色谱（2DGC）现在似乎被保留给 GC×GC 方法。这可以通过分配给第二（D）柱的分析时间来将其与综合多维气相色谱（CMDGC）区分开来，尽管似乎没有一个特定的定义与这个分析时间参数相关。这里描述了几种不同的综合多维气相色谱实现方案，可能涉及单次或多次进样。在所有情况下，必须在每个维度上实现独立保留，以确保增强分离的可能性。本文提出了一种原油样品的原始应用，说明了综合 MDGC 方法的开发，该方法采用两个 Dean 开关（DS）和低温捕集方法，使用顺序心切割（H/C）事件方法，每次进样递增 0.5 分钟；总共进行了 40 次进样来分析总样品。与传统 GC×GC 相比，长 D 柱上的峰容量更高，分辨率更高，并且对石油烃进行了暂定鉴定以进行分类。虽然对于石油烃来说，获取保留指数的方法可能会被实施，但其效用有限。不同化学类别的结构化分组，如原油样品中的单芳烃和二芳烃，被注意到。
