Montone Angela Michela Immacolata, Capuano Federico, Mancusi Andrea, Di Maro Orlandina, Peruzy Maria Francesca, Proroga Yolande Thérèse Rose, Cristiano Daniela
Department of Food Microbiology, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute 2, 80055 Portici (NA), Italy.
Department of Veterinary Medicine and Animal Production, University of Naples "Federico II", Via Delpino 1, 80137 Naples, Italy.
Foods. 2020 Dec 19;9(12):1899. doi: 10.3390/foods9121899.
is a spoilage bacterium and is recognized as an agent of food poisoning. Two food-borne illnesses are caused by : a diarrheal disease, associated with cytotoxin K, hemolysin BL, non-hemolytic enterotoxin and enterotoxin FM, and an emetic syndrome, associated with the cereulide toxin. Owing to the heat resistance of and its ability to grow in milk, this organism should be considered potentially hazardous in dairy products. The present study assessed the risk of poisoning due to the consumption of water buffalo mozzarella cheese. A total of 340 samples were analyzed to determine counts (ISO 7932:2005); isolates underwent molecular characterization to detect the presence of genes encoding toxins. Eighty-nine (26.1%) samples harbored strains, with values ranging from 2.2 × 10 to 2.6 × 10 CFU/g. Isolates showed eight different molecular profiles, and some displayed virulence characteristics. Bacterial counts and the toxin profiles of isolates were evaluated both separately and jointly to assess the risk of enteritis due to following the consumption of buffalo mozzarella cheese. In conclusion, the results of the present study showed that the risk of poisoning by following the consumption of this cheese was moderate.
是一种腐败细菌,被认为是食物中毒的病原体。由它引起两种食源性疾病:一种是腹泻病,与细胞毒素K、溶血素BL、非溶血肠毒素和肠毒素FM有关;另一种是呕吐综合征,与蜡样芽孢杆菌毒素有关。由于该菌具有耐热性且能在牛奶中生长,在乳制品中应被视为潜在危害菌。本研究评估了食用水牛奶酪导致蜡样芽孢杆菌中毒的风险。共分析了340个样本以确定蜡样芽孢杆菌数量(ISO 7932:2005);对分离株进行分子特征分析以检测编码毒素的基因的存在。89个(26.1%)样本含有蜡样芽孢杆菌菌株,数量范围为2.2×10至2.6×10 CFU/g。分离株显示出八种不同的分子谱型,一些具有毒力特征。分别和联合评估分离株的细菌数量和毒素谱型,以评估食用水牛奶酪后由蜡样芽孢杆菌引起肠炎的风险。总之,本研究结果表明,食用这种奶酪后蜡样芽孢杆菌中毒的风险为中度。