INRAE, AgroParisTech, Micalis Institute, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
Sensors (Basel). 2020 May 7;20(9):2667. doi: 10.3390/s20092667.
is an opportunistic foodborne pathogen causing food intoxication and infectious diseases. Different toxins and pathogenic factors are responsible for diarrheal syndrome, like nonhemolytic enterotoxin Nhe, hemolytic enterotoxin Hbl, enterotoxin FM and cytotoxin K, while emetic syndrome is caused by the depsipeptide cereulide toxin. The traditional method of detection is based on the bacterial culturing onto selective agars and cells enumeration. In addition, molecular and chemical methods are proposed for toxin gene profiling, toxin quantification and strain screening for defined virulence factors. Finally, some advanced biosensors such as phage-based, cell-based, immunosensors and DNA biosensors have been elaborated to enable affordable, sensitive, user-friendly and rapid detection of specific strains. This review intends to both illustrate the state of the diagnostic field and to highlight additional research that is still at the development level.
是一种机会性病原体，可导致食物中毒和传染病。不同的毒素和致病因子与腹泻综合征有关，如非溶血肠毒素 Nhe、溶血肠毒素 Hbl、肠毒素 FM 和细胞毒素 K，而呕吐综合征是由环二肽呕吐毒素引起的。传统的检测方法基于细菌在选择性琼脂和细胞上的培养和计数。此外，还提出了分子和化学方法来进行毒素基因分析、毒素定量和针对特定毒力因子的菌株筛选。最后，还详细阐述了一些先进的生物传感器，如噬菌体、细胞、免疫传感器和 DNA 生物传感器，以实现对特定菌株的经济、敏感、用户友好和快速检测。本文旨在阐述诊断领域的现状，并强调仍处于开发阶段的其他研究。
