Olsson J H, Doberl A, Nilsson L
Department of Obstetrics and Gynecology, University of Göteborg, Sweden.
Fertil Steril. 1988 Jan;49(1):42-6.
Danazol concentrations in follicular fluid and serum were studied in eight women scheduled for laparoscopy because of suspected endometriosis. In order to obtain some variation in follicular maturity, danazol administration was started 2 to 7 days before the expected day of ovulation. A total of nine doses were given, i.e., 200 mg four times daily for 2 days; the last 200-mg dose was given 3 hours before the laparoscopy during which the follicular fluid from the dominant follicle was aspirated. Peripheral venous blood samples were drawn before, during, and after laparoscopy. Danazol concentrations were assayed by means of a high-performance liquid chromatography method. At the time of follicular aspiration, the mean concentration of danazol was estimated at 96 ng/ml in serum and at 71 ng/ml in follicular fluid, i.e., an average of 73% of the simultaneous serum concentration. The data suggest that even short-term therapy with danazol is likely to produce intrafollicular drug concentrations that have a direct inhibitory effect on follicular steroidogenesis.
对八名因疑似子宫内膜异位症而计划进行腹腔镜检查的女性，研究了她们卵泡液和血清中的达那唑浓度。为了使卵泡成熟度有所差异，在预计排卵日的前2至7天开始给予达那唑。总共给予九剂，即每天四次，每次200毫克，共2天；最后一剂200毫克在腹腔镜检查前3小时给予，在腹腔镜检查期间抽吸优势卵泡的卵泡液。在腹腔镜检查前、检查期间和检查后采集外周静脉血样本。采用高效液相色谱法测定达那唑浓度。在卵泡抽吸时，血清中达那唑的平均浓度估计为96纳克/毫升，卵泡液中为71纳克/毫升，即平均为同期血清浓度的73%。数据表明，即使是短期使用达那唑治疗，也可能产生对卵泡类固醇生成有直接抑制作用的卵泡内药物浓度。
