Department of Internal Medicine II, University Hospital Würzburg, Würzburg, Germany.
Institute of Pathology, University of Würzburg and Comprehensive Cancer Center (CCC) Mainfranken, Würzburg, Germany.
Leuk Lymphoma. 2021 May;62(5):1107-1115. doi: 10.1080/10428194.2020.1861268. Epub 2020 Dec 22.
In order to differentiate prognostic subgroups of patients with aggressive B-cell lymphoma, we analyzed the expression of 800 miRNAs with the NanoString nCounter human miRNA assay on a cohort of 228 FFPE samples of patients enrolled in the RICOVER-60 and MegaCHOEP trials. We identified significant miRNA signatures for overall survival (OS) and progression-free survival (PFS) by LASSO-penalized linear Cox-regression. High expression levels of miR-130a-3p and miR-423-5p indicate a better prognosis, whereas high levels of miR-374b-5p, miR-590-5p, miR-186-5p, and miR-106b-5p increase patients' risk levels for OS. Regarding PFS high expression of miR-365a-5p in addition to the other two miRNAs improves the prognosis and high levels of miR374a-5p, miR-106b-5p, and miR-590-5p, connects with increased risk and poor prognosis. We identified miRNA signatures to subdivide patients into two different risk groups. These prognostic models may be used in risk stratification in future clinical trials and help making personalized therapy decisions.
为了区分侵袭性 B 细胞淋巴瘤患者的预后亚组，我们使用 NanoString nCounter 人类 miRNA 检测分析了 RICOVER-60 和 MegaCHOEP 试验中 228 例 FFPE 样本中 800 种 miRNA 的表达。我们通过 LASSO 惩罚线性 Cox 回归确定了总生存期 (OS) 和无进展生存期 (PFS) 的显著 miRNA 特征。miR-130a-3p 和 miR-423-5p 的高表达水平表明预后较好，而 miR-374b-5p、miR-590-5p、miR-186-5p 和 miR-106b-5p 的高水平增加了患者 OS 的风险水平。关于 PFS，miR-365a-5p 的高表达水平除了另外两种 miRNA 外，还改善了预后，而 miR-374a-5p、miR-106b-5p 和 miR-590-5p 的高水平与风险增加和预后不良相关。我们确定了 miRNA 特征，可以将患者分为两个不同的风险组。这些预后模型可用于未来临床试验中的风险分层，并有助于做出个体化治疗决策。
