Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen, 6525 GA Nijmegen, The Netherlands.
Department of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen, 6525 GA Nijmegen, The Netherlands.
Trends Biotechnol. 2021 Sep;39(9):890-900. doi: 10.1016/j.tibtech.2020.11.011. Epub 2020 Dec 19.
RNA-protein interactions play an important role in numerous cellular processes in health and disease. In recent years, the global RNA-bound proteome has been extensively studied, uncovering many previously unknown RNA-binding proteins. However, little is known about which particular proteins bind to which specific RNA transcript. In this review, we provide an overview of methods to identify RNA-protein interactions, with a particular focus on strategies that provide insights into the interactome of specific RNA transcripts. Finally, we discuss challenges and future directions, including the potential of CRISPR-RNA targeting systems to investigate endogenous RNA-protein interactions.
RNA-蛋白质相互作用在健康和疾病中的许多细胞过程中起着重要作用。近年来，人们广泛研究了全球 RNA 结合蛋白质组，揭示了许多以前未知的 RNA 结合蛋白。然而，对于哪些特定的蛋白质与哪些特定的 RNA 转录本结合，人们知之甚少。在这篇综述中，我们提供了鉴定 RNA-蛋白质相互作用的方法概述，特别关注提供特定 RNA 转录本相互作用组见解的策略。最后，我们讨论了挑战和未来方向，包括 CRISPR-RNA 靶向系统在研究内源性 RNA-蛋白质相互作用方面的潜力。
