Oh Se Yeon
Department of Food Science & Technology, Keimyung University, Daegu, South Korea.
Phytochem Anal. 2021 Sep;32(5):710-723. doi: 10.1002/pca.3017. Epub 2020 Dec 22.
Korean mugwort has been used as a traditional medicine. Specifically, air-dried Artemisia princeps Pamp. cv. ssajuari (ssajuari-ssuk; Korean mugwort) has been used as moxa in moxibustion therapy. Thus, the evaluation of high quality ssajuari-ssuk containing herbal volatiles is of great interest in clinical therapy and the food industry.
The aim of this study is to determine whether fast gas chromatography with uncoated surface acoustic wave (fast GC/SAW) sensor can be a useful technique for performing quality control using herbal volatiles of ssajuari-ssuk air-dried for different times.
Fast GC/SAW sensor, called "Electronic zNose", is also a headspace sampling method and is an effective simple analytical method with a second unit analysis providing on-line measurements without the need for pretreatment of the sample. Headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) was employed to confirm the identification of the volatiles and compared to fast GC/SAW sensor.
Artemisia princeps Pamp. cv. ssajuari air-dried for 2 years and 4 months (the third year), containing the highest total amount of herbal volatiles, was superior to the others (the first year, the second year, the fourth year) in quality. Moreover, fast GC/SAW sensor shows a high repeatability (relative standard deviation 0.77% ∼ 6.23%), excellent sensitivity (limit of detection value of 0.47 pg/mL), and good linearity (correlation coefficient r = 0.997) over the range of nanogram for α-thujone.
Therefore, the fast GC/SAW sensor can be a useful analytical method for the discrimination and quality control of volatiles of herbal and medicinal plants according to air-drying time, as it provides a simple and second unit analysis.
朝鲜艾已被用作传统药物。具体而言，风干的朝鲜艾蒿变种ssajuari（ssajuari-ssuk；朝鲜艾）已被用作艾灸疗法中的艾绒。因此，对含有草本挥发物的高品质ssajuari-ssuk的评估在临床治疗和食品工业中具有重大意义。
本研究的目的是确定未涂覆表面声波的快速气相色谱法（快速GC/SAW）传感器是否可作为一种有用的技术，用于对不同干燥时间的风干ssajuari-ssuk的草本挥发物进行质量控制。
快速GC/SAW传感器，即“电子鼻”，也是一种顶空进样方法，是一种有效的简单分析方法，其二次分析单元可提供在线测量，无需对样品进行预处理。采用顶空固相微萃取气相色谱-质谱联用（HS-SPME-GC-MS）来确认挥发物的鉴定，并与快速GC/SAW传感器进行比较。
风干2年4个月（第三年）的朝鲜艾蒿变种ssajuari含有最高总量的草本挥发物，在质量上优于其他年份（第一年、第二年、第四年）。此外，快速GC/SAW传感器在α-侧柏酮的纳克范围内显示出高重复性（相对标准偏差0.77%至6.23%）、出色的灵敏度（检测限值为0.47 pg/mL）和良好的线性（相关系数r = 0.997）。
因此，快速GC/SAW传感器可作为一种有用的分析方法，用于根据风干时间对草本和药用植物的挥发物进行鉴别和质量控制，因为它提供了一种简单的二次分析单元。
