Rickmann Annekatrin, Wahl Silke, Katsen-Globa Alisa, Schulz André, Pütz Norbert, Szurman Peter
Eye Clinic Sulzbach, Knappschaft Hospital Saar, Sulzbach, Germany.
Klaus Heimann Eye Research Institute (KHERI), Knappschaft Hospital Saar, Sulzbach, Germany.
Acta Ophthalmol. 2021 Sep;99(6):e937-e942. doi: 10.1111/aos.14716. Epub 2020 Dec 23.
To investigate the ultrastructure of the cleavage plane of human cornea after liquid-bubble-prepared tissue for Descemet's membrane endothelial keratoplasty (DMEK).
Experimental study with scanning electron microscopy (SEM) and block-face SEM of 18 corneal specimens. Fresh human research donor corneoscleral discs (n = 12) were prepared with liquid-bubble technique or examined as untreated controls (n = 3). In addition, Descemet's membrane samples, n = 3, were obtained in DMEK surgery.
The cleavage plane after liquid-bubble Descemet's membrane (DM) preparation was consistently located between interfacial matrix and posterior stromal collagen lamellae, providing a largely smooth surface exposing the amorphous interfacial zone without any significant amounts of adherent stromal remnants. No demarcation of a distinct pre-DM layer could be detected.
The DMEK graft preparation performed by liquid-bubble technique showed a smooth cleavage plane and could not reveal any demarcation of a distinct pre-DM layer.
研究用于后弹力层内皮角膜移植术（DMEK）的液泡制备组织后人类角膜分裂面的超微结构。
对18个角膜标本进行扫描电子显微镜（SEM）和块面SEM的实验研究。用液泡技术制备新鲜人类研究供体角膜巩膜盘（n = 12）或作为未处理对照进行检查（n = 3）。此外，在DMEK手术中获取了3个后弹力层样本。
液泡后弹力层（DM）制备后的分裂面始终位于界面基质和后基质胶原板层之间，提供了一个基本光滑的表面，暴露了无定形界面区，没有任何大量附着的基质残余物。未检测到明显的前DM层的分界。
液泡技术进行的DMEK移植物制备显示出光滑的分裂面，且未发现明显的前DM层的分界。
