Waters Corporation, Milford, Massachusetts, USA.
J Sep Sci. 2021 Mar;44(5):1005-1014. doi: 10.1002/jssc.202001136. Epub 2021 Jan 18.
We have characterized Atlantis ethylene-bridged hybrid C anion-exchange, a mixed-mode reversed-phase/weak anion-exchange stationary phase designed to give greater retention for anions (e.g., ionized acids) compared to conventional reversed-phase materials. The retention and selectivity of this stationary phase were compared to that of three benchmark materials, using a mixture of six polar compounds that includes an acid, two bases, and three neutrals. The compatibility of the ethylene-bridged hybrid C anion-exchange material with 100% aqueous mobile phases was also evaluated. We investigated the batch-to-batch reproducibility of the ethylene-bridged hybrid C anion-exchange stationary phase for 27 batches across three different particle sizes (1.7, 2.5, and 5 μm) and found it to be comparable to that of one of the most reproducible C stationary phases. We also characterized the acid and base stability of the ethylene-bridged hybrid C anion-exchange stationary phase and the results show it to be usable over a wide pH range, from 2 to 10. The extended upper pH limit relative to silica-based reversed-phase/weak anion-exchange materials is enabled by the use of ethylene-bridged hybrid organic/inorganic particles. The improved base stability allows Atlantis ethylene-bridged hybrid C anion-exchange to be used with a wider range of mobile phase pH values, opening up a greater range of selectivity options.
我们对 Atlantis 乙烯桥联混合 C 阴离子交换固定相进行了表征，这是一种混合模式反相/弱阴离子交换固定相，旨在与传统反相材料相比，为阴离子（如离子化酸）提供更大的保留。使用包括一种酸、两种碱和三种中性化合物的六种极性化合物混合物，比较了这种固定相与三种基准材料的保留和选择性。还评估了乙烯桥联混合 C 阴离子交换材料与 100%水性流动相的兼容性。我们研究了乙烯桥联混合 C 阴离子交换固定相在三个不同粒径（1.7、2.5 和 5μm）的 27 个批次之间的批间重现性，发现其与最具重现性的 C 固定相之一相当。我们还对乙烯桥联混合 C 阴离子交换固定相的酸和碱稳定性进行了表征，结果表明它在很宽的 pH 范围内（从 2 到 10）都可用。与基于硅胶的反相/弱阴离子交换材料相比，使用乙烯桥联混合有机/无机颗粒可以实现更宽的 pH 上限。改进的碱稳定性允许 Atlantis 乙烯桥联混合 C 阴离子交换在更宽的流动相 pH 值范围内使用，从而提供更多的选择性选项。
