子宫内膜样腺癌肿瘤细胞中 microRNA 的表达。

Expression of microRNA in tumor cells of endmetrioid carcinoma of endometrium.

机构信息

R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.

National Cancer Institute of the Ministry of Health of Ukraine, Kyiv 03022, Ukraine.

出版信息

Exp Oncol. 2020 Dec;42(4):289-294. doi: 10.32471/exp-oncology.2312-8852.vol-42-no-4.15522.

DOI:10.32471/exp-oncology.2312-8852.vol-42-no-4.15522

PMID:33355860

Abstract

BACKGROUND

It is known that more than half of the genes encoding human proteins are regulated by various microRNAs (miRNAs, miR), the expression of which may be associated with various pathological conditions. At the same time, the question of assessing the relationship between the expression of particular miRNAs and the aggressive molecular subtype of endometrial cancer remains open. Aim of the study was to determine the relationship between the expression of miR-34a, miR-125b, miR-142 and miR-101 in endometrioid carcinomas of the endometrium (ECE) and the features of the disease course.

MATERIALS AND METHODS

The samples of surgical material of 51 patients with ECE (mean age 59.8 ± 7.1 years), I-III stage were investigated using morphological, immunohistochemical methods, real time polymerase chain reaction (PCR), cytofluorometry.

RESULTS

In endometrial tumors with high proliferation index (< Me), the expression of miR-34a, miR-142 and miR-125b significantly decreased (1.8, 2.7 and 1.5 times, respectively) compared with those in ECE with low proliferation index (> Me). The expression of all studied miRNAs was lower in G3 tumors and those that deeply invaded the myometrium compared to G2 carcinomas and tumors with an invasion of > 1/2 myometrium and significantly decreased in tumors of patients with low stage III compared with stage I-II. The high (< Me) microvessel density in ECE was associated with a significant decrease of miR-125b and miR-101 expression, and the presence of signs of epithelial-mesenchymal transition - with a decreased expression of miR-34a and miR-101.

CONCLUSIONS

The study revealed a significant heterogeneity of expression of miR-34a, miR-125b, miR-142 and miR-101 in ECE, which is associated with changes in morphofunctional characteristics of endometrial carcinoma.

摘要

背景

已知，超过一半的人类蛋白质编码基因受各种 microRNAs（miRNAs，miR）调控，这些 miRNAs 的表达可能与各种病理状况相关。同时，特定 miRNAs 的表达与子宫内膜癌侵袭性分子亚型之间的关系评估问题仍然没有答案。本研究旨在确定子宫内膜样癌（ECE）中 miR-34a、miR-125b、miR-142 和 miR-101 的表达与疾病进程特征之间的关系。

材料与方法

使用形态学、免疫组织化学方法、实时聚合酶链反应（PCR）和细胞荧光术，对 51 例 ECE 手术标本（平均年龄 59.8±7.1 岁，I-III 期）进行了检测。

结果

在高增殖指数（<Me）的子宫内膜肿瘤中，miR-34a、miR-142 和 miR-125b 的表达与低增殖指数（>Me）的 ECE 相比显著降低（分别为 1.8、2.7 和 1.5 倍）。与 G2 癌和侵袭>1/2 子宫肌层的肿瘤相比，所有研究的 miRNA 在 G3 肿瘤和深部侵袭子宫肌层的肿瘤中的表达均较低，与 I-II 期相比，III 期低肿瘤患者的肿瘤表达显著降低。ECE 中（<Me）高微血管密度与 miR-125b 和 miR-101 表达的显著降低相关，上皮-间充质转化（epithelial-mesenchymal transition，EMT）的存在与 miR-34a 和 miR-101 表达的降低相关。