Huang Wan-Ling, Ding Li, Yao Jia-Hui, Hu Hang-Tong, Gao Yong, Xie Xiao-Yan, Lu Ming-De, Deng Chun-Hua, Xie Yun, Wang Zhu
Department of Medical Ultrasonics, Institute of Diagnostic and Interventional Ultrasound, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
Department of Pathology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China.
Andrologia. 2021 Mar;53(2):e13927. doi: 10.1111/and.13927. Epub 2020 Dec 23.
Busulfan-induced testicular injury mouse models are commonly used for experiments on spermatogonial stem cell transplantation, treatments for azoospermia due to spermatogenic failure and preserving male fertility after chemotherapy. Here, we investigated the value of testicular quantitative ultrasound for evaluating spermatogenic function in this model. In this study, testicular ultrasound was performed on mice from day 0 to 126 after busulfan treatment (n = 48), and quantitative data, including the testicular volume, mean pixel intensity and pixel uniformity, were analysed. The results revealed that from day 0 to 36, the testicular volume was positively associated with the testicle-to-body weight ratio (r = .92). On day 63, the pixel uniformity, which remained stable from day 0 to 36, declined significantly compared with that on day 36 (p < .01). On day 126, when the whole progression of spermatogenesis could be observed in most tubules, the mean pixel intensity also returned to normal (p > .05). In conclusion, testicular quantitative ultrasound could be used as a noninvasive and accurate monitoring method for evaluating spermatogenic function in busulfan-induced testicular injury mouse models.
白消安诱导的睾丸损伤小鼠模型常用于精原干细胞移植实验、治疗生精功能衰竭所致无精子症以及化疗后保留男性生育能力。在此，我们研究了睾丸定量超声在评估该模型生精功能方面的价值。在本研究中，对接受白消安治疗后第0天至126天的小鼠进行睾丸超声检查（n = 48），并分析包括睾丸体积、平均像素强度和像素均匀性在内的定量数据。结果显示，从第0天至36天，睾丸体积与睾丸 - 体重比呈正相关（r = 0.92）。在第63天，从第0天至36天一直保持稳定的像素均匀性与第36天相比显著下降（p < 0.01）。在第126天，当在大多数小管中可观察到整个生精过程时，平均像素强度也恢复正常（p > 0.05）。总之，睾丸定量超声可作为一种无创且准确的监测方法，用于评估白消安诱导的睾丸损伤小鼠模型中的生精功能。
