Campus Macaé, Universidade Federal do Rio de Janeiro , Macaé, Brazil.
Instituto de Puericultura e Pediatria Martagão Gesteira, Universidade Federal do Rio de Janeiro , Rio de Janeiro, Brazil.
Virulence. 2021 Dec;12(1):260-269. doi: 10.1080/21505594.2020.1869484.
Atopic dermatitis (AD) is a chronic inflammatory skin disease and colonization by may affect up to 100% of these patients. Virulent and resistant isolates can worsen AD patient clinical condition and jeopardize the treatment. We aimed to detect virulence genes and to evaluate the biofilm production of isolates from infected skin lesions of children with AD. Methicillin resistance was detected by phenotypic and molecular tests and the virulence genes were detected by PCR. Biofilm formation was assessed by bacterial growing on microtiter plates and later stained with safranin. Genotyping was performed by Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing. Among 106 AD patients, 55 (51.8%) had developed cutaneous infections and 23 (41.6%) were methicillin-resistant (MRSA). All 55 isolates carried the and genes, and more than 70% presented and genes. Clonal complex (CC) 30 was the main lineage found (34.5%), especially among MRSA isolates (52.2%). The cluster and the gene were significantly the most frequent in MRSA isolates and in USA1100/ST30/CC30 lineage. Most of the isolates (74.5%) were non-biofilm producers and many of them only started to produce it in the presence of fibrinogen. There was no significant association between isolates features and the AD severity. This study demonstrated a high frequency of CC30 MRSA isolates presenting several virulence genes in infected skin lesions of AD children in Brazil, that may influence the severity of the disease and the treatments required.
特应性皮炎（AD）是一种慢性炎症性皮肤病，金黄色葡萄球菌定植可影响高达 100%的这些患者。毒力和耐药分离株可使 AD 患者的临床状况恶化，并危及治疗。我们旨在检测金黄色葡萄球菌分离株的毒力基因，并评估其生物膜的产生。通过表型和分子试验检测耐甲氧西林金黄色葡萄球菌，通过 PCR 检测毒力基因。通过在微量滴定板上生长细菌并随后用沙黄染色来评估生物膜形成。通过脉冲场凝胶电泳和多位点序列分型进行基因分型。在 106 例 AD 患者中，55 例（51.8%）发生了皮肤感染，23 例（41.6%）为耐甲氧西林金黄色葡萄球菌（MRSA）。所有 55 株分离株均携带 和 基因，超过 70%的分离株携带 和 基因。发现的主要谱系是克隆复合体（CC）30（34.5%），尤其是在 MRSA 分离株中（52.2%）。 簇和 基因是 MRSA 分离株和 USA1100/ST30/CC30 谱系中最常见的基因。大多数分离株（74.5%）是非生物膜生产者，其中许多分离株仅在存在纤维蛋白原时才开始产生生物膜。金黄色葡萄球菌分离株的特征与 AD 严重程度之间没有显著相关性。本研究表明，巴西 AD 儿童感染部位的 CC30 MRSA 分离株携带多种毒力基因的频率较高，这可能影响疾病的严重程度和所需的治疗方法。
