Eye Institute, Affiliated Hospital of Nantong University, Nantong 226000, China.
Eye Institute, Affiliated Hospital of Nantong University, Nantong 226000, China.
Chin J Nat Med. 2020 Dec;18(12):934-940. doi: 10.1016/S1875-5364(20)60037-1.
A contributory role of oxidative stress and protection by antioxidant nutrients have been suspected in cataract formation. Ganoderic acid A (GAA), an effective lanostane triterpene, is widely reported as an antioxidant. The aim of this study is to investigate the potential effects of GAA on cataract formation. After lens epithelial cells (LECs) were exposed to UVB radiation for different periods, cell viability, apoptosis-related protein levels, malondialdehyde (MDA) and superoxide dismutase (SOD) activities were monitored. We found that cell viability, the Bcl-2/Bax ratio and SOD activity were increased, while Cleaved caspase-3 levels and MDA activity were decreased compared with those in UVB-impaired LECs after GAA treated. Furthermore, GAA activated PI3K/AKT in UVB-impaired LECs and effectively delayed the occurrence of lens opacity in vitro. In conclusion, these findings demonstrated that GAA exhibited protective functions in SRA01/04 cells and rat lenses against UVB-evoked impairment through elevating cell viability and antioxidant activity, inhibiting cell apoptosis, activating the PI3K/AKT pathway and delaying lens opacity.
氧化应激的促成作用以及抗氧化营养物质的保护作用已被怀疑与白内障的形成有关。灵芝酸 A (GAA) 是一种有效的羊毛甾烷三萜，被广泛报道为一种抗氧化剂。本研究旨在探讨 GAA 对白内障形成的潜在影响。在 Lens 上皮细胞 (LECs) 暴露于不同时间的 UVB 辐射后，监测细胞活力、与细胞凋亡相关的蛋白水平、丙二醛 (MDA) 和超氧化物歧化酶 (SOD) 活性。我们发现，与 UVB 损伤的 LECs 相比，用 GAA 处理后，细胞活力、Bcl-2/Bax 比值和 SOD 活性增加，而 Cleaved caspase-3 水平和 MDA 活性降低。此外，GAA 在 UVB 损伤的 LECs 中激活了 PI3K/AKT，并有效地延缓了体外晶状体混浊的发生。总之，这些发现表明，GAA 通过提高细胞活力和抗氧化活性、抑制细胞凋亡、激活 PI3K/AKT 通路和延缓晶状体混浊，对 SRA01/04 细胞和大鼠晶状体的 UVB 诱导损伤表现出保护作用。
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