Department of Orthopedic Surgery, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori, Japan.
Department of Orthopedic Surgery, Hirosaki University Graduate School of Medicine, Hirosaki, Aomori, Japan.
J Pharmacol Sci. 2021 Jan;145(1):23-28. doi: 10.1016/j.jphs.2020.10.003. Epub 2020 Oct 13.
Ossification of the posterior longitudinal ligament (OPLL) within the spinal canal sometimes leads to severe myelopathy. Teriparatide (TPD) is a recombinant human parathyroid hormone (PTH) (1-34), which promotes osteogenesis of mesenchymal stem cells (MSCs) via PTH 1 receptor (PTH1R). Although ligamentum flavum (LF)-MSCs from patients with OPLL have a high osteogenic potency, the effect of TPD on them remains unknown. In this study, we determined PTH1R expression in LF-MSCs from patients with OPLL and investigated whether TPD promotes osteogenic differentiation in them. First, LF-MSCs were isolated from patients with OPLL and cervical spondylotic myelopathy (CSM) (controls). Cultured LF-MSCs were treated with different concentrations of TPD on days 0, 7, and 14. On day 21, osteogenic gene expression was quantified. Mineralization was measured based on optical density after Alizarin Red S staining. LF-MSCs from both groups expressed PTH1R at the same level. TPD did not enhance osteogenic gene expression and mineralization in LF-MSCs from both groups. TPD did not promote the osteogenic differentiation of LF-MSCs from patients with OPLL. Thus, it may be safe for patients with OPLL. However, further confirmation of our results with in vivo studies is necessary.
椎管内的后纵韧带骨化（OPLL）有时会导致严重的脊髓病。特立帕肽（TPD）是一种重组人甲状旁腺激素（PTH）（1-34），通过甲状旁腺素 1 受体（PTH1R）促进间充质干细胞（MSCs）的成骨作用。虽然来自 OPLL 患者的黄韧带（LF）-MSCs 具有较高的成骨潜能，但 TPD 对其的影响尚不清楚。在这项研究中，我们确定了 OPLL 患者 LF-MSCs 中的 PTH1R 表达，并研究了 TPD 是否促进它们的成骨分化。首先，从 OPLL 和颈椎病（CSM）（对照组）患者中分离 LF-MSCs。在第 0、7 和 14 天，用不同浓度的 TPD 处理培养的 LF-MSCs。第 21 天，定量测定成骨基因表达。根据茜素红 S 染色后的光密度测量矿化。两组 LF-MSCs 均表达相同水平的 PTH1R。TPD 并未增强两组 LF-MSCs 的成骨基因表达和矿化。TPD 并未促进 OPLL 患者 LF-MSCs 的成骨分化。因此，它对 OPLL 患者可能是安全的。然而，需要通过体内研究进一步证实我们的结果。
