黑麦草的综合处理：半纤维素的结构特征分析及纤维素酶解性能的改善。
Integrated treatment of perennial ryegrass: Structural characterization of hemicelluloses and improvement of enzymatic hydrolysis of cellulose.
机构信息
Key Laboratory for Forest Resources Conservation and Utilisation in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, 650224, PR China.
The Key Laboratory of State Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming, 650224, PR China.
出版信息
Carbohydr Polym. 2021 Feb 15;254:117257. doi: 10.1016/j.carbpol.2020.117257. Epub 2020 Oct 19.
An integrated treatment coupling ultrasonic and hydrothermal pretreatments with sequential alkali post-extractions was performed to isolate and characterize hemicelluloses from perennial ryegrass and improve the enzymatic hydrolysis efficiency of cellulose. The yield, chemical composition, and structure of water-soluble and alkali-soluble hemicelluloses obtained from the hydrothermal supernatant and hydrothermally pretreated ryegrass as well as the enzymatic hydrolysis efficiency of cellulose were comprehensively investigated by gel permeation chromatograph, high-performance anion exchange chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectra. Results showed that more than 90 % of the original hemicelluloses in ryegrass were released during the integrated treatment and all hemicellulosic fractions obtained were mainly composed of ʟ-arabino-(4-O-methyl-ᴅ-glucurono)-ᴅ-xylans, galactoanrabinoxylans and β-glucans. In addition, the effective removal of amorphous hemicelluloses and lignin significantly increased the cellulose enzymatic hydrolysis rate of ryegrass from 43.8 to 91.1 %. These results provided new insights into the collaborative utilization of hemicelluloses and cellulose in ryegrass.
采用超声和水热预处理与顺序碱后提取相结合的综合处理方法，从多年生黑麦草中分离和表征半纤维素，并提高纤维素的酶水解效率。通过凝胶渗透色谱、高效阴离子交换色谱、傅里叶变换红外光谱和核磁共振谱，综合研究了水热处理上清液和水热预处理黑麦草中获得的水溶性和碱溶性半纤维素的产率、化学成分和结构，以及纤维素的酶水解效率。结果表明，在综合处理过程中，黑麦草中超过 90%的原始半纤维素被释放出来，所有得到的半纤维素级分主要由 L-阿拉伯糖-(4-O-甲基-ᴅ-葡萄糖醛酸)-D-木聚糖、半乳糖阿拉伯木聚糖和β-葡聚糖组成。此外，无定形半纤维素和木质素的有效去除显著提高了黑麦草纤维素的酶水解率，从 43.8%提高到 91.1%。这些结果为黑麦草中半纤维素和纤维素的协同利用提供了新的见解。
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