在念珠菌属中，基因型与毒力之间缺乏关系。

Lack of relationship between genotype and virulence in Candida species.

机构信息

Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Universidad Complutense de Madrid, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.

Unit of Mycology, Statens Serum Institut, Copenhagen, Denmark; Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark; Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark.

出版信息

Rev Iberoam Micol. 2021 Jan-Mar;38(1):9-11. doi: 10.1016/j.riam.2020.10.002. Epub 2021 Jan 7.

DOI:10.1016/j.riam.2020.10.002

PMID:33358083

Abstract

BACKGROUND

The virulence of isolates among different Candida species causing candidemia may play a role in the prognosis of the patients. Furthermore, the potential relationship between genotype and virulence is still unclear and need to be further studied.

AIMS

We aim to assess the relationship between genotype and virulence in Candida species using a Galleria mellonella larvae infection model.

METHODS

One hundred and ninety-four isolates from 68 clusters (Candida albicans, 114/41; Candida parapsilosis, 74/24; Candida tropicalis, 6/3) were compared against the same number of each species singleton genotypes in terms of survival of G. mellonella larvae.

RESULTS

The median of survival and the IQR ranges of clusters and singleton were as follows: C. albicans (2 days, IQR 1.5-2 vs. 2 days, IQR 1-2.25), C. parapsilosis (2 days, IQR 1.5-2.6 vs. 2 days, IQR 2-3.3), and C. tropicalis (1 day, IQR 1-3.5 vs. 2 days, IQR 2-3.5; p<0.05). High intra-cluster variability in terms of median of survival was found regardless the species.

CONCLUSIONS

No relationship between genotype and virulence in Candida was observed with the G. mellonella model.

摘要

背景

引起菌血症的不同念珠菌种的分离株的毒力可能在患者的预后中起作用。此外，基因型和毒力之间的潜在关系尚不清楚，需要进一步研究。

目的

我们旨在使用大蜡螟幼虫感染模型评估念珠菌属种中基因型和毒力之间的关系。

方法

将 68 个群（白色念珠菌 114/41；近平滑念珠菌 74/24；热带念珠菌 6/3）的 194 株分离株与每个种的相同数量的单倍型基因型进行比较，以评估大蜡螟幼虫的存活率。

结果

群和单倍型的中位生存时间和 IQR 范围如下：白色念珠菌（2 天，IQR 1.5-2 与 2 天，IQR 1-2.25）、近平滑念珠菌（2 天，IQR 1.5-2.6 与 2 天，IQR 2-3.3）和热带念珠菌（1 天，IQR 1-3.5 与 2 天，IQR 2-3.5；p<0.05）。无论物种如何，群内中位生存时间的变异性都很高。